This Jupyter notebook is intented to be used alongside the book Python for Bioinformatics
Note: The code in this chapter needs Biopython 1.69. It was not included in the first print of the book because this Biopython version was not available. The full source code in the GitHub repository. This version is adapted to work in Jupyter Notebook.
In [2]:
!pip install biopython
Collecting biopython
Downloading biopython-1.70.tar.gz (15.7MB)
100% |################################| 15.7MB 89kB/s eta 0:00:01
Requirement already satisfied: numpy in /home/nbcommon/anaconda3_431/lib/python3.6/site-packages (from biopython)
Building wheels for collected packages: biopython
Running setup.py bdist_wheel for biopython ... - \ | / - \ | / - \ | / - \ | / - \ | done
Stored in directory: /home/nbuser/.cache/pip/wheels/b6/3d/13/02803d941d2488e0920c7f482ddebe949cb6f346d9fe205bf9
Successfully built biopython
Installing collected packages: biopython
Successfully installed biopython-1.70
In [10]:
'''Given a DNA sequence of a polypeptide, this program
generates alternative DNA sequences that code for the same
polypeptide but that can be sorted out by DNA restriction.
Requires Biopython >= 1.69
Author: Sebastian Bassi (sbassi@genesdigitales.com)'''
from Bio import Seq
from Bio.Alphabet import IUPAC
from Bio import Restriction
from Bio.Data import CodonTable
from jinja2 import Template
TABLE_ID = 1
ALLOWED_MUTATIONS = 2
mutation_tpl = """Original Sequence: {{dna_input}}
Peptide: {{ori_pep}}
Restriction map for original sequence:
{{ori_map}}
=========================
{% for item in bakpeps_out %}
Original sequence enzymes: {{oname}}
{{item.graph}}
Proposed sequence enzymes: {{item.pames}}
Enzimes only in original sequence: {{item.o}}
Enzimes only in proposed sequence: {{item.p}}
=========================
{% endfor %}"""
def backtrans(ori_pep, table_id=1):
"""
Function to make backtranslation (from peptide to DNA)
This function needs the peptide sequence and the code of
translation table. Code number is the same as posted in:
http://www.ncbi.nlm.nih.gov/Taxonomy/Utils/wprintgc.cgi
"""
def recurs(order, pos):
for letter in bt[order[pos]]:
if pos == len(order) - 1:
yield letter
continue
for prox in recurs(order, pos+1):
yield (letter + prox)
def combine(order):
ordened = set()
for frase in recurs(order, 0):
ordened.add(frase)
return ordened
t = CodonTable.generic_by_id[table_id]
bt = dict()
for a1 in "ATCG" :
for a2 in "ATCG" :
for a3 in "ATCG" :
codon = a1 + a2 + a3
try:
amino = t.forward_table[codon]
except KeyError:
assert codon in t.stop_codons
continue
try:
bt[amino].append(codon)
except KeyError:
bt[amino] = [codon]
return list(combine(ori_pep))
def seqcomp(s1, s2):
"""
Compares 2 sequences and returns a value with
how many differents elements they have.
"""
p = len(s1)
for x,y in zip(s1, s2): # Walk through 2 sequences.
if x==y:
p -= 1
return p
input_dna = input('Enter DNA sequennce: ').upper()
dna = Seq.Seq(input_dna, IUPAC.unambiguous_dna)
# Translate DNA sequence.
ori_pep = dna.translate()
# Get all backtranslations.
bakpeps = backtrans(ori_pep, TABLE_ID)
# Make a restriction analysis for the orignal sequence.
analysis = Restriction.Analysis(Restriction.CommOnly, dna)
analysis.print_as('map')
ori_map = analysis.format_output()
# Store the enzymes that cut in the original sequence.
enz = list(analysis.with_sites().keys())
# Get a string out of the enzyme list, for printing.
oname = str(enz)[1:-1]
enz = set(enz)
bakpeps_out = []
for bakpep in bakpeps:
tmp_d = {}
if bakpep not in input_dna:
# Make a restriction analysis for each sequence.
analysis = Restriction.Analysis(Restriction.CommOnly,
Seq.Seq(bakpep, IUPAC.unambiguous_dna))
# Store the enzymes that cut in this sequence.
enz_tmp = list(analysis.with_sites().keys())
enz_tmp = set(enz_tmp)
# Get the number of mutations in backpep sequence.
y = seqcomp(input_dna, bakpep)
if enz_tmp != enz and enz and y <= ALLOWED_MUTATIONS:
analysis.print_as('map')
tmp_d['pames'] = str(enz_tmp)[1:-1]
tmp_d['graph'] = analysis.format_output()
tmp_d['ori_seq'] = str(list(enz.difference(
enz_tmp)))[1:-1]
tmp_d['proposed_seq'] = str(list(
enz_tmp.difference(enz)))[1:-1]
bakpeps_out.append(tmp_d)
template = Template(mutation_tpl)
render = template.render(dna_input=input_dna,
ori_pep=ori_pep,
ori_map=ori_map,
oname=oname,
bakpeps_out=bakpeps_out)
print(render)
Enter DNA sequennce: atacgctgtcatgca
Original Sequence: ATACGCTGTCATGCA
Peptide: IRCHA
Restriction map for original sequence:
4 AbaSI
|
| 10 FatI
| |
| |11 CviAII
| ||
| ||12 FaiI
| |||
| ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| ||| |
ATACGCTGTCATGCA
|||||||||||||||
TATGCGACAGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI
MboI MluCI MnlI MseI MspI MspJI MvnI NdeII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI BisI
BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I
BseBI BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI
BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI
BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI
Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI
HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I
LmnI Lsp1109I LweI MaeIII MboII MlyI MspR9I MvaI
NciI NmuCI PfeI PkrI PleI PpsI Psp6I PspGI
PspPI SatI Sau96I SchI ScrFI SfaNI SinI StyD4I
TaaI TauI TfiI TscAI TseFI TseI Tsp45I TspDTI
TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I
AccB1I AccBSI AccI AccIII AclI AcoI AcsI AcuI
AcvI AcyI AfeI AflII AflIII AgeI AhlI AjiI
Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI
AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII
BaeGI BalI BamHI BanI BanII BauI BbrPI BbsI
Bbv12I BciVI BclI BcuI BfmI BfoI BfrI BfuAI
BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI
BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI
BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI
BseDI BseMI BsePI BseRI BseSI BseX3I BseYI BsgI
Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI
BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I
Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI
BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI
BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI
BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI
BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI
BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI
BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I
CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI
EaeI EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I
Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI
Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I
EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI FblI
FriOI FspI GsaI GsuI HaeII Hin1I HincII HindII
HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I
KpnI KroI Ksp22I KspAI KspI MbiI MfeI MflI
MhlI MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I
MroI MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I
NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII
NruI NsbI NsiI NspI NspV PaeI PaeR7I PagI
PauI PceI PciI PctI PdiI Pfl23II PinAI Ple19I
PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI
Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI PsuI
PteI PvuI PvuII RruI SacI SacII SalI ScaI
SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI
SmaI SmlI SmoI SnaBI SpeI SphI SseBI SspDI
SspI SstI StuI StyI TaqII TatI TspMI Vha464I
VneI VspI XapI XbaI XceI XhoI XmaI XmaJI
XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI
Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I
CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI
LguI MabI PasI PciSI PfoI PpuMI Psp5II PspEI
PspPPI Rsr2I RsrII SapI SexAI AbsI AscI AsiSI
CciNI FseI FspAI MauBI MreI MssI NotI PacI
PalAI PmeI PspXI RgaI RigI SbfI SdaI SfaAI
SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI
AdeI AlwNI CaiI DraIII MteI PflFI PstNI PsyI
Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI
MroXI MslI OliI PdmI PshAI RseI SmiMI XmnI
AccB7I AfiI AhdI BaeI BglI BmeRI BplI BsaXI
Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I
EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI AasI
BcgI BstXI CspCI DrdI DseDI AloI ArsI BarI
PcsI PsrI SfiI AjuI XcmI
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AciI AbaSI BspACI SsiI
|
| 6 MspA1I
| |
| | 8 TseFI NmuCI Tsp45I MaeIII
| | |
| | | 15 AbaSI
| | | |
ATCCGCTGTCACGCA
|||||||||||||||
TAGGCGACAGTGCGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AfaI AluBI AluI AoxI AspLEI BfaI
BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspANI BspFNI
BssMI BstFNI BstHHI BstKTI BstMBI BstUI BsuRI CfoI
Csp6I CviAII CviJI CviKI_1 CviQI DpnI DpnII FaeI
FaiI FatI FspBI GlaI HaeIII HapII HhaI Hin1II
Hin6I HinP1I HpaII HpyCH4IV HpyCH4V HpySE526I Hsp92II HspAI
Kzo9I LpnPI MaeI MaeII MalI MboI MluCI MnlI
MseI MspI MspJI MvnI NdeII NlaIII RsaI RsaNI
SaqAI Sau3AI SetI SgeI Sse9I SspMI TaiI TaqI
TasI Tru1I Tru9I XspI AclWI AgsI AjnI Alw26I
AlwI ApeKI AspS9I AsuC2I AsuHPI AvaII BbvI BccI
BceAI BciT130I BcnI BcoDI BisI BlsI Bme1390I Bme18I
BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI BseMII
BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI BsrI
Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI BstV1I
BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII FaqI
FauI Fnu4HI FokI Fsp4HI GluI HgaI HinfI HphI
Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI
MboII MlyI MspR9I MvaI NciI PfeI PkrI PleI
PpsI Psp6I PspGI PspPI SatI Sau96I SchI ScrFI
SfaNI SinI StyD4I TaaI TauI TfiI TscAI TseI
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspCI MunI Mva1269I
NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII
NruI NsbI NsiI NspI NspV PaeI PaeR7I PagI
PauI PceI PciI PctI PdiI Pfl23II PinAI Ple19I
PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI
Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI PsuI
PteI PvuI PvuII RruI SacI SacII SalI ScaI
SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI
SmaI SmlI SmoI SnaBI SpeI SphI SseBI SspDI
SspI SstI StuI StyI TaqII TatI TspMI Vha464I
VneI VspI XapI XbaI XceI XhoI XmaI XmaJI
XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI
Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I
CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI
LguI MabI PasI PciSI PfoI PpuMI Psp5II PspEI
PspPPI Rsr2I RsrII SapI SexAI AbsI AscI AsiSI
CciNI FseI FspAI MauBI MreI MssI NotI PacI
PalAI PmeI PspXI RgaI RigI SbfI SdaI SfaAI
SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI
AdeI AlwNI CaiI DraIII MteI PflFI PstNI PsyI
Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI
MroXI MslI OliI PdmI PshAI RseI SmiMI XmnI
AccB7I AfiI AhdI BaeI BglI BmeRI BplI BsaXI
Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I
EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI AasI
BcgI BstXI CspCI DrdI DseDI AloI ArsI BarI
PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: BspACI, TseFI, AbaSI, NmuCI, Tsp45I, MaeIII, AciI, SsiI, MspA1I
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 ApeKI TseI
| |
| |7 GluI Fsp4HI Fnu4HI SatI BisI
| ||
| ||8 PkrI BlsI
| |||
| ||| 10 FatI
| ||| |
| ||| |11 CviAII
| ||| ||
| ||| ||12 FaiI
| ||| |||
| ||| ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| ||| ||| |
ATACGCTGCCATGCA
|||||||||||||||
TATGCGACGGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI
MboI MluCI MnlI MseI MspI MspJI MvnI NdeII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI AspS9I AsuC2I AsuHPI AvaII
BbvI BccI BceAI BciT130I BcnI BcoDI Bme1390I Bme18I
BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI BseMII
BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI BsrI
Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI BstV1I
BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII FaqI
FauI FokI HgaI HinfI HphI Hpy188I Hpy99I HpyAV
HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI
MspR9I MvaI NciI NmuCI PfeI PleI PpsI Psp6I
PspGI PspPI Sau96I SchI ScrFI SfaNI SinI StyD4I
TaaI TauI TfiI TscAI TseFI Tsp45I TspDTI TspGWI
TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I
AccBSI AccI AccIII AclI AcoI AcsI AcuI AcvI
AcyI AfeI AflII AflIII AgeI AhlI AjiI Alw21I
Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI
AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI
BalI BamHI BanI BanII BauI BbrPI BbsI Bbv12I
BciVI BclI BcuI BfmI BfoI BfrI BfuAI BfuI
BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI
BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI
BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI
BseMI BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I
BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI
BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I
Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI
BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI
BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I
Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI
BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI
BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI
BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I CciI
Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI
EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I
Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I
Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I
EgeI EheI ErhI Esp3I FauNDI FbaI FblI FriOI
FspI GsaI GsuI HaeII Hin1I HincII HindII HindIII
HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI
KroI Ksp22I KspAI KspI MbiI MfeI MflI MhlI
MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI
MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI
NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI
NsbI NsiI NspI NspV PaeI PaeR7I PagI PauI
PceI PciI PctI PdiI Pfl23II PinAI Ple19I PluTI
PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I
PspCI PspFI PspLI PspN4I PspOMI PstI PsuI PteI
PvuI PvuII RruI SacI SacII SalI ScaI SduI
SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI
SmlI SmoI SnaBI SpeI SphI SseBI SspDI SspI
SstI StuI StyI TaqII TatI TspMI Vha464I VneI
VspI XapI XbaI XceI XhoI XmaI XmaJI XmiI
ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I
Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI
CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI
MabI PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI
Rsr2I RsrII SapI SexAI AbsI AscI AsiSI CciNI
FseI FspAI MauBI MreI MssI NotI PacI PalAI
PmeI PspXI RgaI RigI SbfI SdaI SfaAI SgfI
SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI
AlwNI CaiI DraIII MteI PflFI PstNI PsyI Tth111I
AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI
MslI OliI PdmI PshAI RseI SmiMI XmnI AccB7I
AfiI AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I
BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI
FalI HpyF10VI MwoI PflMI Van91I XagI AasI BcgI
BstXI CspCI DrdI DseDI AloI ArsI BarI PcsI
PsrI SfiI AjuI XcmI
Proposed sequence enzymes: TseI, SatI, GluI, AbaSI, Fsp4HI, CviAII, FaiI, FaeI, FatI, HpyCH4V, Fnu4HI, PkrI, Hsp92II, ApeKI, BisI, BlsI, NlaIII, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 10 FatI
| |
| |11 CviAII
| ||
| ||12 FaiI
| |||
| ||| 14 Hin1II NlaIII FaeI Hsp92II
| ||| |
ATTCGCTGTCATGCC
|||||||||||||||
TAAGCGACAGTACGG
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I
BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI
DdeI Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI
GluI HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III
HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI MspR9I
MvaI NciI NmuCI PfeI PkrI PleI PpsI Psp6I
PspGI PspPI SatI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI TseI Tsp45I
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspA1I MspCI MunI
Mva1269I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV
NmeAIII NruI NsbI NsiI NspI NspV PaeI PaeR7I
PagI PauI PceI PciI PctI PdiI Pfl23II PinAI
Ple19I PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI
Psp124BI Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI
PsuI PteI PvuI PvuII RruI SacI SacII SalI
ScaI SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI
SlaI SmaI SmlI SmoI SnaBI SpeI SphI SseBI
SspDI SspI SstI StuI StyI TaqII TatI TspMI
Vha464I VneI VspI XapI XbaI XceI XhoI XmaI
XmaJI XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI
BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI
Bsu36I CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I
KflI LguI MabI PasI PciSI PfoI PpuMI Psp5II
PspEI PspPPI Rsr2I RsrII SapI SexAI AbsI AscI
AsiSI CciNI FseI FspAI MauBI MreI MssI NotI
PacI PalAI PmeI PspXI RgaI RigI SbfI SdaI
SfaAI SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I
SwaI AdeI AlwNI CaiI DraIII MteI PflFI PstNI
PsyI Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI
BstPAI MroXI MslI OliI PdmI PshAI RseI SmiMI
XmnI AccB7I AfiI AhdI BaeI BglI BmeRI BplI
BsaXI Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI
Eam1105I EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI
AasI BcgI BstXI CspCI DrdI DseDI AloI ArsI
BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 MaeII AbaSI HpyCH4IV HpySE526I
|
| 7 TaiI SetI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| | ||| |
ATACGTTGCCATGCA
|||||||||||||||
TATGCAACGGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HspAI Kzo9I LpnPI MaeI MalI MboI MluCI MnlI
MseI MspI MspJI MvnI NdeII RsaI RsaNI SaqAI
Sau3AI SgeI Sse9I SsiI SspMI TaqI TasI Tru1I
Tru9I XspI AclWI AgsI AjnI Alw26I AlwI ApeKI
AspS9I AsuC2I AsuHPI AvaII BbvI BccI BceAI BciT130I
BcnI BcoDI BisI BlsI Bme1390I Bme18I BmgT120I BmrFI
BmsI BpuMI Bse1I BseBI BseGI BseMII BseNI BseXI
BslFI BsmAI BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI
BstDEI BstF5I BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI
Cfr13I CseI DdeI Eco47I EcoRII FaqI FauI Fnu4HI
FokI Fsp4HI GluI HgaI HinfI HphI Hpy188I Hpy99I
HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII MboII
MlyI MspR9I MvaI NciI NmuCI PfeI PkrI PleI
PpsI Psp6I PspGI PspPI SatI Sau96I SchI ScrFI
SfaNI SinI StyD4I TaaI TauI TfiI TscAI TseFI
TseI Tsp45I TspDTI TspGWI TspRI VpaK11BI AanI AatII
Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII AclI
AcoI AcsI AcuI AcvI AcyI AfeI AflII AflIII
AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI
ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I AsuII
AsuNHI AvaI AvrII BaeGI BalI BamHI BanI BanII
BauI BbrPI BbsI Bbv12I BciVI BclI BcuI BfmI
BfoI BfrI BfuAI BfuI BglII BlnI BmcAI BmeT110I
BmgBI BmiI BmrI BmtI BmuI BpiI BpmI Bpu14I
BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI Bse118I
Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI BseSI
BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI BsiEI
BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I
Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI
BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I BspTI
BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI BssHII
BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI
BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I BstMCI
BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I
BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI BtsI
BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI
CspAI DinI DraI EaeI EagI Eam1104I EarI EciI
Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I
Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI
EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI Esp3I
FauNDI FbaI FblI FriOI FspI GsaI GsuI HaeII
Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III Hpy8I
Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI KspI
MbiI MfeI MflI MhlI MlsI MluI MluNI Mly113I
MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I MspA1I
MspCI MunI Mva1269I NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NmeAIII NruI NsbI NsiI NspI NspV
PaeI PaeR7I PagI PauI PceI PciI PctI PdiI
Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I PscI
PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI PspN4I
PspOMI PstI PsuI PteI PvuI PvuII RruI SacI
SacII SalI ScaI SduI SfcI SfoI Sfr274I Sfr303I
SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI SpeI
SphI SseBI SspDI SspI SstI StuI StyI TaqII
TatI TspMI Vha464I VneI VspI XapI XbaI XceI
XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I AarI
AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI
BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I Eco91I
EcoO109I EcoO65I KflI LguI MabI PasI PciSI PfoI
PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI SexAI
AbsI AscI AsiSI CciNI FseI FspAI MauBI MreI
MssI NotI PacI PalAI PmeI PspXI RgaI RigI
SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI SmiI
SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII MteI
PflFI PstNI PsyI Tth111I AleI Asp700I BoxI BsaBI
Bse8I BseJI BstPAI MroXI MslI OliI PdmI PshAI
RseI SmiMI XmnI AccB7I AfiI AhdI BaeI BglI
BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI BstENI
BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI PflMI
Van91I XagI AasI BcgI BstXI CspCI DrdI DseDI
AloI ArsI BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: MaeII, NlaIII, AbaSI, CviAII, HpyCH4IV, TaiI, FaeI, HpySE526I, FaiI, FatI, Hsp92II, HpyCH4V, SetI, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AciI AbaSI BspACI SsiI
|
| 6 AbaSI MspA1I
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
| | ||| |15 AbaSI
| | ||| ||
ATCCGCTGTCATGCG
|||||||||||||||
TAGGCGACAGTACGC
1 15
Enzymes which do not cut the sequence.
FspEI AccII AfaI AluBI AluI AoxI AspLEI BfaI
BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspANI BspFNI
BssMI BstFNI BstHHI BstKTI BstMBI BstUI BsuRI CfoI
Csp6I CviJI CviKI_1 CviQI DpnI DpnII FspBI GlaI
HaeIII HapII HhaI Hin6I HinP1I HpaII HpyCH4IV HpyCH4V
HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI MboI
MluCI MnlI MseI MspI MspJI MvnI NdeII RsaI
RsaNI SaqAI Sau3AI SetI SgeI Sse9I SspMI TaiI
TaqI TasI Tru1I Tru9I XspI AclWI AgsI AjnI
Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI AvaII BbvI
BccI BceAI BciT130I BcnI BcoDI BisI BlsI Bme1390I
Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI
BseMII BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI
BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI
BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII
FaqI FauI Fnu4HI FokI Fsp4HI GluI HgaI HinfI
HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I LmnI Lsp1109I
LweI MaeIII MboII MlyI MspR9I MvaI NciI NmuCI
PfeI PkrI PleI PpsI Psp6I PspGI PspPI SatI
Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI TauI
TfiI TscAI TseFI TseI Tsp45I TspDTI TspGWI TspRI
VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I AccBSI
AccI AccIII AclI AcoI AcsI AcuI AcvI AcyI
AfeI AflII AflIII AgeI AhlI AjiI Alw21I Alw44I
Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI AsiGI
Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI BalI
BamHI BanI BanII BauI BbrPI BbsI Bbv12I BciVI
BclI BcuI BfmI BfoI BfrI BfuAI BfuI BglII
BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI BmuI
BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI
BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI BseMI
BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I BshNI
BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI
Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I
Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI BspOI
BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI BsrGI
BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I Bst1107I
Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI BstC8I
BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I
BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI
BtgZI BtrI BtsI BtuMI BveI Cac8I CciI Cfr10I
Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI EagI
Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I Eco147I
Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I
Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI
EheI ErhI Esp3I FauNDI FbaI FblI FriOI FspI
GsaI GsuI HaeII Hin1I HincII HindII HindIII HpaI
Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI KroI
Ksp22I KspAI KspI MbiI MfeI MflI MhlI MlsI
MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI MroNI
MscI Msp20I MspCI MunI Mva1269I NaeI NarI NcoI
NdeI NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI
NspI NspV PaeI PaeR7I PagI PauI PceI PciI
PctI PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI
Ppu21I PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI
PspLI PspN4I PspOMI PstI PsuI PteI PvuI PvuII
RruI SacI SacII SalI ScaI SduI SfcI SfoI
Sfr274I Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI
SnaBI SpeI SphI SseBI SspDI SspI SstI StuI
StyI TaqII TatI TspMI Vha464I VneI VspI XapI
XbaI XceI XhoI XmaI XmaJI XmiI ZraI ZrmI
Zsp2I AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I
Bsp1720I BspQI BstEII BstPI Bsu36I CpoI CsiI CspI
Eco81I Eco91I EcoO109I EcoO65I KflI LguI MabI PasI
PciSI PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII
SapI SexAI AbsI AscI AsiSI CciNI FseI FspAI
MauBI MreI MssI NotI PacI PalAI PmeI PspXI
RgaI RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI
SgsI SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI
DraIII MteI PflFI PstNI PsyI Tth111I AleI Asp700I
BoxI BsaBI Bse8I BseJI BstPAI MroXI MslI OliI
PdmI PshAI RseI SmiMI XmnI AccB7I AfiI AhdI
BaeI BglI BmeRI BplI BsaXI Bsc4I BseLI BslI
BstAPI BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI
MwoI PflMI Van91I XagI AasI BcgI BstXI CspCI
DrdI DseDI AloI ArsI BarI PcsI PsrI SfiI
AjuI XcmI
Proposed sequence enzymes: BspACI, AbaSI, NlaIII, CviAII, FaiI, FatI, AciI, FaeI, SsiI, Hsp92II, MspA1I, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 TaqI AbaSI
|
| 10 FatI
| |
| |11 CviAII
| ||
| ||12 FaiI
| |||
| ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| ||| |
ATTCGATGTCATGCA
|||||||||||||||
TAAGCTACAGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI
MboI MluCI MnlI MseI MspI MspJI MvnI NdeII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TasI Tru1I Tru9I XspI AclWI AgsI
AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI AvaII
BbvI BccI BceAI BciT130I BcnI BcoDI BisI BlsI
Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I BseBI
BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI BspCNI
BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI
BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I
EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI HgaI
HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I LmnI
Lsp1109I LweI MaeIII MboII MlyI MspR9I MvaI NciI
NmuCI PfeI PkrI PleI PpsI Psp6I PspGI PspPI
SatI Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI
TauI TfiI TscAI TseFI TseI Tsp45I TspDTI TspGWI
TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I
AccBSI AccI AccIII AclI AcoI AcsI AcuI AcvI
AcyI AfeI AflII AflIII AgeI AhlI AjiI Alw21I
Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI
AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI
BalI BamHI BanI BanII BauI BbrPI BbsI Bbv12I
BciVI BclI BcuI BfmI BfoI BfrI BfuAI BfuI
BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI
BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI
BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI
BseMI BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I
BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI
BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I
Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI
BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI
BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I
Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI
BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI
BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI
BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I CciI
Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI
EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I
Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I
Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I
EgeI EheI ErhI Esp3I FauNDI FbaI FblI FriOI
FspI GsaI GsuI HaeII Hin1I HincII HindII HindIII
HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI
KroI Ksp22I KspAI KspI MbiI MfeI MflI MhlI
MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI
MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI
NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI
NsbI NsiI NspI NspV PaeI PaeR7I PagI PauI
PceI PciI PctI PdiI Pfl23II PinAI Ple19I PluTI
PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I
PspCI PspFI PspLI PspN4I PspOMI PstI PsuI PteI
PvuI PvuII RruI SacI SacII SalI ScaI SduI
SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI
SmlI SmoI SnaBI SpeI SphI SseBI SspDI SspI
SstI StuI StyI TaqII TatI TspMI Vha464I VneI
VspI XapI XbaI XceI XhoI XmaI XmaJI XmiI
ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I
Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI
CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI
MabI PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI
Rsr2I RsrII SapI SexAI AbsI AscI AsiSI CciNI
FseI FspAI MauBI MreI MssI NotI PacI PalAI
PmeI PspXI RgaI RigI SbfI SdaI SfaAI SgfI
SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI
AlwNI CaiI DraIII MteI PflFI PstNI PsyI Tth111I
AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI
MslI OliI PdmI PshAI RseI SmiMI XmnI AccB7I
AfiI AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I
BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI
FalI HpyF10VI MwoI PflMI Van91I XagI AasI BcgI
BstXI CspCI DrdI DseDI AloI ArsI BarI PcsI
PsrI SfiI AjuI XcmI
Proposed sequence enzymes: TaqI, AbaSI, CviAII, FaiI, FatI, FaeI, HpyCH4V, Hsp92II, NlaIII, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AciI AbaSI BspACI SsiI
|
| 6 MspA1I
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
| | ||| |15 AbaSI
| | ||| ||
ATCCGCTGTCATGCT
|||||||||||||||
TAGGCGACAGTACGA
1 15
Enzymes which do not cut the sequence.
FspEI AccII AfaI AluBI AluI AoxI AspLEI BfaI
BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspANI BspFNI
BssMI BstFNI BstHHI BstKTI BstMBI BstUI BsuRI CfoI
Csp6I CviJI CviKI_1 CviQI DpnI DpnII FspBI GlaI
HaeIII HapII HhaI Hin6I HinP1I HpaII HpyCH4IV HpyCH4V
HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI MboI
MluCI MnlI MseI MspI MspJI MvnI NdeII RsaI
RsaNI SaqAI Sau3AI SetI SgeI Sse9I SspMI TaiI
TaqI TasI Tru1I Tru9I XspI AclWI AgsI AjnI
Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI AvaII BbvI
BccI BceAI BciT130I BcnI BcoDI BisI BlsI Bme1390I
Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI
BseMII BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI
BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI
BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII
FaqI FauI Fnu4HI FokI Fsp4HI GluI HgaI HinfI
HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I LmnI Lsp1109I
LweI MaeIII MboII MlyI MspR9I MvaI NciI NmuCI
PfeI PkrI PleI PpsI Psp6I PspGI PspPI SatI
Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI TauI
TfiI TscAI TseFI TseI Tsp45I TspDTI TspGWI TspRI
VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I AccBSI
AccI AccIII AclI AcoI AcsI AcuI AcvI AcyI
AfeI AflII AflIII AgeI AhlI AjiI Alw21I Alw44I
Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI AsiGI
Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI BalI
BamHI BanI BanII BauI BbrPI BbsI Bbv12I BciVI
BclI BcuI BfmI BfoI BfrI BfuAI BfuI BglII
BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI BmuI
BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI
BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI BseMI
BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I BshNI
BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI
Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I
Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI BspOI
BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI BsrGI
BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I Bst1107I
Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI BstC8I
BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I
BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI
BtgZI BtrI BtsI BtuMI BveI Cac8I CciI Cfr10I
Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI EagI
Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I Eco147I
Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I
Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI
EheI ErhI Esp3I FauNDI FbaI FblI FriOI FspI
GsaI GsuI HaeII Hin1I HincII HindII HindIII HpaI
Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI KroI
Ksp22I KspAI KspI MbiI MfeI MflI MhlI MlsI
MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI MroNI
MscI Msp20I MspCI MunI Mva1269I NaeI NarI NcoI
NdeI NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI
NspI NspV PaeI PaeR7I PagI PauI PceI PciI
PctI PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI
Ppu21I PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI
PspLI PspN4I PspOMI PstI PsuI PteI PvuI PvuII
RruI SacI SacII SalI ScaI SduI SfcI SfoI
Sfr274I Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI
SnaBI SpeI SphI SseBI SspDI SspI SstI StuI
StyI TaqII TatI TspMI Vha464I VneI VspI XapI
XbaI XceI XhoI XmaI XmaJI XmiI ZraI ZrmI
Zsp2I AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I
Bsp1720I BspQI BstEII BstPI Bsu36I CpoI CsiI CspI
Eco81I Eco91I EcoO109I EcoO65I KflI LguI MabI PasI
PciSI PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII
SapI SexAI AbsI AscI AsiSI CciNI FseI FspAI
MauBI MreI MssI NotI PacI PalAI PmeI PspXI
RgaI RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI
SgsI SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI
DraIII MteI PflFI PstNI PsyI Tth111I AleI Asp700I
BoxI BsaBI Bse8I BseJI BstPAI MroXI MslI OliI
PdmI PshAI RseI SmiMI XmnI AccB7I AfiI AhdI
BaeI BglI BmeRI BplI BsaXI Bsc4I BseLI BslI
BstAPI BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI
MwoI PflMI Van91I XagI AasI BcgI BstXI CspCI
DrdI DseDI AloI ArsI BarI PcsI PsrI SfiI
AjuI XcmI
Proposed sequence enzymes: BspACI, AbaSI, NlaIII, CviAII, FaiI, FatI, AciI, FaeI, SsiI, Hsp92II, MspA1I, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 AbaSI ApeKI TseI
| |
| |7 GluI Fsp4HI Fnu4HI SatI BisI
| ||
| ||8 PkrI BlsI
| |||
| ||| 10 FatI
| ||| |
| ||| |11 CviAII
| ||| ||
| ||| ||12 FaiI
| ||| |||
| ||| ||| 14 Hin1II NlaIII FaeI Hsp92II
| ||| ||| |
ATACGCTGCCATGCG
|||||||||||||||
TATGCGACGGTACGC
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI Bme1390I
Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI
BseMII BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI
BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI
BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII
FaqI FauI FokI HgaI HinfI HphI Hpy188I Hpy99I
HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII MboII
MlyI MspR9I MvaI NciI NmuCI PfeI PleI PpsI
Psp6I PspGI PspPI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI Tsp45I TspDTI
TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I
AccB1I AccBSI AccI AccIII AclI AcoI AcsI AcuI
AcvI AcyI AfeI AflII AflIII AgeI AhlI AjiI
Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI
AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII
BaeGI BalI BamHI BanI BanII BauI BbrPI BbsI
Bbv12I BciVI BclI BcuI BfmI BfoI BfrI BfuAI
BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI
BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI
BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI
BseDI BseMI BsePI BseRI BseSI BseX3I BseYI BsgI
Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI
BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I
Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI
BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI
BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI
BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI
BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI
BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI
BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I
CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI
EaeI EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I
Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI
Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I
EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI FblI
FriOI FspI GsaI GsuI HaeII Hin1I HincII HindII
HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I
KpnI KroI Ksp22I KspAI KspI MbiI MfeI MflI
MhlI MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I
MroI MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I
NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII
NruI NsbI NsiI NspI NspV PaeI PaeR7I PagI
PauI PceI PciI PctI PdiI Pfl23II PinAI Ple19I
PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI
Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI PsuI
PteI PvuI PvuII RruI SacI SacII SalI ScaI
SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI
SmaI SmlI SmoI SnaBI SpeI SphI SseBI SspDI
SspI SstI StuI StyI TaqII TatI TspMI Vha464I
VneI VspI XapI XbaI XceI XhoI XmaI XmaJI
XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI
Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I
CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI
LguI MabI PasI PciSI PfoI PpuMI Psp5II PspEI
PspPPI Rsr2I RsrII SapI SexAI AbsI AscI AsiSI
CciNI FseI FspAI MauBI MreI MssI NotI PacI
PalAI PmeI PspXI RgaI RigI SbfI SdaI SfaAI
SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI
AdeI AlwNI CaiI DraIII MteI PflFI PstNI PsyI
Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI
MroXI MslI OliI PdmI PshAI RseI SmiMI XmnI
AccB7I AfiI AhdI BaeI BglI BmeRI BplI BsaXI
Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I
EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI AasI
BcgI BstXI CspCI DrdI DseDI AloI ArsI BarI
PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: TseI, GluI, AbaSI, Fsp4HI, CviAII, FaiI, FaeI, FatI, Fnu4HI, PkrI, SatI, Hsp92II, ApeKI, BisI, BlsI, NlaIII, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 AbaSI
| |
| | 8 TseFI NmuCI Tsp45I MaeIII
| | |
| | | 14 BstUI BstFNI AccII MvnI BspFNI Bsh1236I
| | | |
ATACGCTGTCACGCG
|||||||||||||||
TATGCGACAGTGCGC
1 15
Enzymes which do not cut the sequence.
FspEI AciI AfaI AluBI AluI AoxI AspLEI BfaI
BfuCI BshFI BsiSI BsnI Bsp143I BspACI BspANI BssMI
BstHHI BstKTI BstMBI BsuRI CfoI Csp6I CviAII CviJI
CviKI_1 CviQI DpnI DpnII FaeI FaiI FatI FspBI
GlaI HaeIII HapII HhaI Hin1II Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I Hsp92II HspAI Kzo9I LpnPI MaeI
MaeII MalI MboI MluCI MnlI MseI MspI MspJI
NdeII NlaIII RsaI RsaNI SaqAI Sau3AI SetI SgeI
Sse9I SsiI SspMI TaiI TaqI TasI Tru1I Tru9I
XspI AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I
AsuC2I AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI
BcoDI BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI
BpuMI Bse1I BseBI BseGI BseMII BseNI BseXI BslFI
BsmAI BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI
BstF5I BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I
CseI DdeI Eco47I EcoRII FaqI FauI Fnu4HI FokI
Fsp4HI GluI HgaI HinfI HphI Hpy188I Hpy99I HpyAV
HpyCH4III HpyF3I LmnI Lsp1109I LweI MboII MlyI MspR9I
MvaI NciI PfeI PkrI PleI PpsI Psp6I PspGI
PspPI SatI Sau96I SchI ScrFI SfaNI SinI StyD4I
TaaI TauI TfiI TscAI TseI TspDTI TspGWI TspRI
VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I AccBSI
AccI AccIII AclI AcoI AcsI AcuI AcvI AcyI
AfeI AflII AflIII AgeI AhlI AjiI Alw21I Alw44I
Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI AsiGI
Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI BalI
BamHI BanI BanII BauI BbrPI BbsI Bbv12I BciVI
BclI BcuI BfmI BfoI BfrI BfuAI BfuI BglII
BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI BmuI
BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI
BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI BseMI
BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I BshNI
BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI
Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I
Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI BspOI
BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI BsrGI
BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I Bst1107I
Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI BstC8I
BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I
BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI
BtgZI BtrI BtsI BtuMI BveI Cac8I CciI Cfr10I
Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI EagI
Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I Eco147I
Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I
Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI
EheI ErhI Esp3I FauNDI FbaI FblI FriOI FspI
GsaI GsuI HaeII Hin1I HincII HindII HindIII HpaI
Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI KroI
Ksp22I KspAI KspI MbiI MfeI MflI MhlI MlsI
MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI MroNI
MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI NsbI
NsiI NspI NspV PaeI PaeR7I PagI PauI PceI
PciI PctI PdiI Pfl23II PinAI Ple19I PluTI PmaCI
PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I PspCI
PspFI PspLI PspN4I PspOMI PstI PsuI PteI PvuI
PvuII RruI SacI SacII SalI ScaI SduI SfcI
SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI SmlI
SmoI SnaBI SpeI SphI SseBI SspDI SspI SstI
StuI StyI TaqII TatI TspMI Vha464I VneI VspI
XapI XbaI XceI XhoI XmaI XmaJI XmiI ZraI
ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I Bpu1102I
Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI CsiI
CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI MabI
PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI Rsr2I
RsrII SapI SexAI AbsI AscI AsiSI CciNI FseI
FspAI MauBI MreI MssI NotI PacI PalAI PmeI
PspXI RgaI RigI SbfI SdaI SfaAI SgfI SgrAI
SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI AlwNI
CaiI DraIII MteI PflFI PstNI PsyI Tth111I AleI
Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI MslI
OliI PdmI PshAI RseI SmiMI XmnI AccB7I AfiI
AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I BseLI
BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI FalI
HpyF10VI MwoI PflMI Van91I XagI AasI BcgI BstXI
CspCI DrdI DseDI AloI ArsI BarI PcsI PsrI
SfiI AjuI XcmI
Proposed sequence enzymes: TseFI, MvnI, BstFNI, BstUI, NmuCI, AbaSI, BspFNI, Tsp45I, MaeIII, Bsh1236I, AccII
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 BanI BshNI AccB1I HpyCH4III Bst4CI TaaI BspT107I
| |
| | 8 BspLI PspN4I BmiI NlaIV
| | |
| | | 10 FatI
| | | |
| | | |11 CviAII
| | | ||
| | | ||12 FaiI
| | | |||
| | | ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| | | ||| |
ATACGGTGCCATGCA
|||||||||||||||
TATGCCACGGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI
MboI MluCI MnlI MseI MspI MspJI MvnI NdeII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI BisI
BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I
BseBI BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI
BspCNI BspPI BsrI Bst2UI BstDEI BstF5I BstMAI BstNI
BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I
EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI HgaI
HinfI HphI Hpy188I Hpy99I HpyAV HpyF3I LmnI Lsp1109I
LweI MaeIII MboII MlyI MspR9I MvaI NciI NmuCI
PfeI PkrI PleI PpsI Psp6I PspGI PspPI SatI
Sau96I SchI ScrFI SfaNI SinI StyD4I TauI TfiI
TscAI TseFI TseI Tsp45I TspDTI TspGWI TspRI VpaK11BI
AanI AatII Acc16I Acc36I Acc65I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanII
BauI BbrPI BbsI Bbv12I BciVI BclI BcuI BfmI
BfoI BfrI BfuAI BfuI BglII BlnI BmcAI BmeT110I
BmgBI BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI
Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI
BseAI BseCI BseDI BseMI BsePI BseRI BseSI BseX3I
BseYI BsgI Bsh1285I BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspMAI
BspMI BspOI BspT104I BspTI BspTNI BsrBI BsrDI BsrFI
BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I
Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI
BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI
BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI
BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I CciI
Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI
EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I
Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I
Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I
EgeI EheI ErhI Esp3I FauNDI FbaI FblI FriOI
FspI GsaI GsuI HaeII Hin1I HincII HindII HindIII
HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI
KroI Ksp22I KspAI KspI MbiI MfeI MflI MhlI
MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI
MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI
NarI NcoI NdeI NgoMIV NheI NmeAIII NruI NsbI
NsiI NspI NspV PaeI PaeR7I PagI PauI PceI
PciI PctI PdiI Pfl23II PinAI Ple19I PluTI PmaCI
PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I PspCI
PspFI PspLI PspOMI PstI PsuI PteI PvuI PvuII
RruI SacI SacII SalI ScaI SduI SfcI SfoI
Sfr274I Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI
SnaBI SpeI SphI SseBI SspDI SspI SstI StuI
StyI TaqII TatI TspMI Vha464I VneI VspI XapI
XbaI XceI XhoI XmaI XmaJI XmiI ZraI ZrmI
Zsp2I AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I
Bsp1720I BspQI BstEII BstPI Bsu36I CpoI CsiI CspI
Eco81I Eco91I EcoO109I EcoO65I KflI LguI MabI PasI
PciSI PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII
SapI SexAI AbsI AscI AsiSI CciNI FseI FspAI
MauBI MreI MssI NotI PacI PalAI PmeI PspXI
RgaI RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI
SgsI SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI
DraIII MteI PflFI PstNI PsyI Tth111I AleI Asp700I
BoxI BsaBI Bse8I BseJI BstPAI MroXI MslI OliI
PdmI PshAI RseI SmiMI XmnI AccB7I AfiI AhdI
BaeI BglI BmeRI BplI BsaXI Bsc4I BseLI BslI
BstAPI BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI
MwoI PflMI Van91I XagI AasI BcgI BstXI CspCI
DrdI DseDI AloI ArsI BarI PcsI PsrI SfiI
AjuI XcmI
Proposed sequence enzymes: Bst4CI, FatI, BmiI, BanI, NlaIV, BspLI, NlaIII, AbaSI, CviAII, BshNI, FaeI, PspN4I, AccB1I, FaiI, HpyCH4III, Hsp92II, HpyCH4V, BspT107I, Hin1II, TaaI
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 10 FatI
| |
| |11 CviAII
| ||
| ||12 FaiI
| |||
| ||| 14 Hin1II NlaIII FaeI Hsp92II
| ||| |
ATACGCTGTCATGCT
|||||||||||||||
TATGCGACAGTACGA
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I
BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI
DdeI Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI
GluI HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III
HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI MspR9I
MvaI NciI NmuCI PfeI PkrI PleI PpsI Psp6I
PspGI PspPI SatI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI TseI Tsp45I
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspA1I MspCI MunI
Mva1269I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV
NmeAIII NruI NsbI NsiI NspI NspV PaeI PaeR7I
PagI PauI PceI PciI PctI PdiI Pfl23II PinAI
Ple19I PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI
Psp124BI Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI
PsuI PteI PvuI PvuII RruI SacI SacII SalI
ScaI SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI
SlaI SmaI SmlI SmoI SnaBI SpeI SphI SseBI
SspDI SspI SstI StuI StyI TaqII TatI TspMI
Vha464I VneI VspI XapI XbaI XceI XhoI XmaI
XmaJI XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI
BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI
Bsu36I CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I
KflI LguI MabI PasI PciSI PfoI PpuMI Psp5II
PspEI PspPPI Rsr2I RsrII SapI SexAI AbsI AscI
AsiSI CciNI FseI FspAI MauBI MreI MssI NotI
PacI PalAI PmeI PspXI RgaI RigI SbfI SdaI
SfaAI SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I
SwaI AdeI AlwNI CaiI DraIII MteI PflFI PstNI
PsyI Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI
BstPAI MroXI MslI OliI PdmI PshAI RseI SmiMI
XmnI AccB7I AfiI AhdI BaeI BglI BmeRI BplI
BsaXI Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI
Eam1105I EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI
AasI BcgI BstXI CspCI DrdI DseDI AloI ArsI
BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AciI AbaSI BspACI SsiI
|
| 6 MspA1I
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| | ||| |
| | ||| |15 AbaSI
| | ||| ||
ATCCGCTGTCATGCA
|||||||||||||||
TAGGCGACAGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AfaI AluBI AluI AoxI AspLEI BfaI
BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspANI BspFNI
BssMI BstFNI BstHHI BstKTI BstMBI BstUI BsuRI CfoI
Csp6I CviJI CviKI_1 CviQI DpnI DpnII FspBI GlaI
HaeIII HapII HhaI Hin6I HinP1I HpaII HpyCH4IV HpySE526I
HspAI Kzo9I LpnPI MaeI MaeII MalI MboI MluCI
MnlI MseI MspI MspJI MvnI NdeII RsaI RsaNI
SaqAI Sau3AI SetI SgeI Sse9I SspMI TaiI TaqI
TasI Tru1I Tru9I XspI AclWI AgsI AjnI Alw26I
AlwI ApeKI AspS9I AsuC2I AsuHPI AvaII BbvI BccI
BceAI BciT130I BcnI BcoDI BisI BlsI Bme1390I Bme18I
BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI BseMII
BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI BsrI
Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI BstV1I
BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII FaqI
FauI Fnu4HI FokI Fsp4HI GluI HgaI HinfI HphI
Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI
MaeIII MboII MlyI MspR9I MvaI NciI NmuCI PfeI
PkrI PleI PpsI Psp6I PspGI PspPI SatI Sau96I
SchI ScrFI SfaNI SinI StyD4I TaaI TauI TfiI
TscAI TseFI TseI Tsp45I TspDTI TspGWI TspRI VpaK11BI
AanI AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI
AccIII AclI AcoI AcsI AcuI AcvI AcyI AfeI
AflII AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I
Aor13HI Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I
AspA2I AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI
BanI BanII BauI BbrPI BbsI Bbv12I BciVI BclI
BcuI BfmI BfoI BfrI BfuAI BfuI BglII BlnI
BmcAI BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI
BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI
BsaWI Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI
BseRI BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI
BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I
BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I
BspDI BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I
BspT107I BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI
BssECI BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI
Bst6I BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI
BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I
BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI
BtrI BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I
Cfr9I ClaI CspAI DinI DraI EaeI EagI Eam1104I
EarI EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I
Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I
EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI
ErhI Esp3I FauNDI FbaI FblI FriOI FspI GsaI
GsuI HaeII Hin1I HincII HindII HindIII HpaI Hpy166II
Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I
KspAI KspI MbiI MfeI MflI MhlI MlsI MluI
MluNI Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI
Msp20I MspCI MunI Mva1269I NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI NspI
NspV PaeI PaeR7I PagI PauI PceI PciI PctI
PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I
PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI
PspN4I PspOMI PstI PsuI PteI PvuI PvuII RruI
SacI SacII SalI ScaI SduI SfcI SfoI Sfr274I
Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI
SpeI SphI SseBI SspDI SspI SstI StuI StyI
TaqII TatI TspMI Vha464I VneI VspI XapI XbaI
XceI XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I
AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I
BspQI BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I
Eco91I EcoO109I EcoO65I KflI LguI MabI PasI PciSI
PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI
SexAI AbsI AscI AsiSI CciNI FseI FspAI MauBI
MreI MssI NotI PacI PalAI PmeI PspXI RgaI
RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI
SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII
MteI PflFI PstNI PsyI Tth111I AleI Asp700I BoxI
BsaBI Bse8I BseJI BstPAI MroXI MslI OliI PdmI
PshAI RseI SmiMI XmnI AccB7I AfiI AhdI BaeI
BglI BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI
BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI
PflMI Van91I XagI AasI BcgI BstXI CspCI DrdI
DseDI AloI ArsI BarI PcsI PsrI SfiI AjuI
XcmI
Proposed sequence enzymes: BspACI, AbaSI, NlaIII, CviAII, FaiI, FatI, AciI, FaeI, HpyCH4V, SsiI, Hsp92II, MspA1I, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AciI AbaSI BspACI SsiI
|
| 6 MspA1I
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
| | ||| |15 AbaSI
| | ||| ||
ATCCGCTGTCATGCC
|||||||||||||||
TAGGCGACAGTACGG
1 15
Enzymes which do not cut the sequence.
FspEI AccII AfaI AluBI AluI AoxI AspLEI BfaI
BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspANI BspFNI
BssMI BstFNI BstHHI BstKTI BstMBI BstUI BsuRI CfoI
Csp6I CviJI CviKI_1 CviQI DpnI DpnII FspBI GlaI
HaeIII HapII HhaI Hin6I HinP1I HpaII HpyCH4IV HpyCH4V
HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI MboI
MluCI MnlI MseI MspI MspJI MvnI NdeII RsaI
RsaNI SaqAI Sau3AI SetI SgeI Sse9I SspMI TaiI
TaqI TasI Tru1I Tru9I XspI AclWI AgsI AjnI
Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI AvaII BbvI
BccI BceAI BciT130I BcnI BcoDI BisI BlsI Bme1390I
Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI
BseMII BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI
BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI
BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII
FaqI FauI Fnu4HI FokI Fsp4HI GluI HgaI HinfI
HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I LmnI Lsp1109I
LweI MaeIII MboII MlyI MspR9I MvaI NciI NmuCI
PfeI PkrI PleI PpsI Psp6I PspGI PspPI SatI
Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI TauI
TfiI TscAI TseFI TseI Tsp45I TspDTI TspGWI TspRI
VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I AccBSI
AccI AccIII AclI AcoI AcsI AcuI AcvI AcyI
AfeI AflII AflIII AgeI AhlI AjiI Alw21I Alw44I
Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI AsiGI
Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI BalI
BamHI BanI BanII BauI BbrPI BbsI Bbv12I BciVI
BclI BcuI BfmI BfoI BfrI BfuAI BfuI BglII
BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI BmuI
BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI
BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI BseMI
BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I BshNI
BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI
Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I
Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI BspOI
BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI BsrGI
BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I Bst1107I
Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI BstC8I
BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I
BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI
BtgZI BtrI BtsI BtuMI BveI Cac8I CciI Cfr10I
Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI EagI
Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I Eco147I
Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I
Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI
EheI ErhI Esp3I FauNDI FbaI FblI FriOI FspI
GsaI GsuI HaeII Hin1I HincII HindII HindIII HpaI
Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI KroI
Ksp22I KspAI KspI MbiI MfeI MflI MhlI MlsI
MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI MroNI
MscI Msp20I MspCI MunI Mva1269I NaeI NarI NcoI
NdeI NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI
NspI NspV PaeI PaeR7I PagI PauI PceI PciI
PctI PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI
Ppu21I PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI
PspLI PspN4I PspOMI PstI PsuI PteI PvuI PvuII
RruI SacI SacII SalI ScaI SduI SfcI SfoI
Sfr274I Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI
SnaBI SpeI SphI SseBI SspDI SspI SstI StuI
StyI TaqII TatI TspMI Vha464I VneI VspI XapI
XbaI XceI XhoI XmaI XmaJI XmiI ZraI ZrmI
Zsp2I AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I
Bsp1720I BspQI BstEII BstPI Bsu36I CpoI CsiI CspI
Eco81I Eco91I EcoO109I EcoO65I KflI LguI MabI PasI
PciSI PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII
SapI SexAI AbsI AscI AsiSI CciNI FseI FspAI
MauBI MreI MssI NotI PacI PalAI PmeI PspXI
RgaI RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI
SgsI SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI
DraIII MteI PflFI PstNI PsyI Tth111I AleI Asp700I
BoxI BsaBI Bse8I BseJI BstPAI MroXI MslI OliI
PdmI PshAI RseI SmiMI XmnI AccB7I AfiI AhdI
BaeI BglI BmeRI BplI BsaXI Bsc4I BseLI BslI
BstAPI BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI
MwoI PflMI Van91I XagI AasI BcgI BstXI CspCI
DrdI DseDI AloI ArsI BarI PcsI PsrI SfiI
AjuI XcmI
Proposed sequence enzymes: BspACI, AbaSI, NlaIII, CviAII, FaiI, FatI, AciI, FaeI, SsiI, Hsp92II, MspA1I, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 8 SetI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| | ||| |
ATAAGGTGTCATGCA
|||||||||||||||
TATTCCACAGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI
MboI MluCI MnlI MseI MspI MspJI MvnI NdeII
RsaI RsaNI SaqAI Sau3AI SgeI Sse9I SsiI SspMI
TaiI TaqI TasI Tru1I Tru9I XspI AclWI AgsI
AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI AvaII
BbvI BccI BceAI BciT130I BcnI BcoDI BisI BlsI
Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I BseBI
BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI BspCNI
BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI
BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I
EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI HgaI
HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I LmnI
Lsp1109I LweI MaeIII MboII MlyI MspR9I MvaI NciI
NmuCI PfeI PkrI PleI PpsI Psp6I PspGI PspPI
SatI Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI
TauI TfiI TscAI TseFI TseI Tsp45I TspDTI TspGWI
TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I
AccBSI AccI AccIII AclI AcoI AcsI AcuI AcvI
AcyI AfeI AflII AflIII AgeI AhlI AjiI Alw21I
Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI
AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI
BalI BamHI BanI BanII BauI BbrPI BbsI Bbv12I
BciVI BclI BcuI BfmI BfoI BfrI BfuAI BfuI
BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI
BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI
BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI
BseMI BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I
BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI
BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I
Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI
BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI
BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I
Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI
BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI
BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI
BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I CciI
Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI
EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I
Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I
Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I
EgeI EheI ErhI Esp3I FauNDI FbaI FblI FriOI
FspI GsaI GsuI HaeII Hin1I HincII HindII HindIII
HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI
KroI Ksp22I KspAI KspI MbiI MfeI MflI MhlI
MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI
MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI
NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI
NsbI NsiI NspI NspV PaeI PaeR7I PagI PauI
PceI PciI PctI PdiI Pfl23II PinAI Ple19I PluTI
PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I
PspCI PspFI PspLI PspN4I PspOMI PstI PsuI PteI
PvuI PvuII RruI SacI SacII SalI ScaI SduI
SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI
SmlI SmoI SnaBI SpeI SphI SseBI SspDI SspI
SstI StuI StyI TaqII TatI TspMI Vha464I VneI
VspI XapI XbaI XceI XhoI XmaI XmaJI XmiI
ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I
Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI
CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI
MabI PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI
Rsr2I RsrII SapI SexAI AbsI AscI AsiSI CciNI
FseI FspAI MauBI MreI MssI NotI PacI PalAI
PmeI PspXI RgaI RigI SbfI SdaI SfaAI SgfI
SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI
AlwNI CaiI DraIII MteI PflFI PstNI PsyI Tth111I
AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI
MslI OliI PdmI PshAI RseI SmiMI XmnI AccB7I
AfiI AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I
BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI
FalI HpyF10VI MwoI PflMI Van91I XagI AasI BcgI
BstXI CspCI DrdI DseDI AloI ArsI BarI PcsI
PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, HpyCH4V, Hsp92II, SetI, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 HpyCH4III Bst4CI TaaI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
ATACGGTGTCATGCC
|||||||||||||||
TATGCCACAGTACGG
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI BstDEI BstF5I BstMAI
BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI
Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI
HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyF3I LmnI
Lsp1109I LweI MaeIII MboII MlyI MspR9I MvaI NciI
NmuCI PfeI PkrI PleI PpsI Psp6I PspGI PspPI
SatI Sau96I SchI ScrFI SfaNI SinI StyD4I TauI
TfiI TscAI TseFI TseI Tsp45I TspDTI TspGWI TspRI
VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I AccBSI
AccI AccIII AclI AcoI AcsI AcuI AcvI AcyI
AfeI AflII AflIII AgeI AhlI AjiI Alw21I Alw44I
Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI AsiGI
Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI BalI
BamHI BanI BanII BauI BbrPI BbsI Bbv12I BciVI
BclI BcuI BfmI BfoI BfrI BfuAI BfuI BglII
BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI BmuI
BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI
BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI BseMI
BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I BshNI
BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI
Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I
Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI BspOI
BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI BsrGI
BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I Bst1107I
Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI BstC8I
BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I
BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI
BtgZI BtrI BtsI BtuMI BveI Cac8I CciI Cfr10I
Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI EagI
Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I Eco147I
Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I
Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI
EheI ErhI Esp3I FauNDI FbaI FblI FriOI FspI
GsaI GsuI HaeII Hin1I HincII HindII HindIII HpaI
Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI KroI
Ksp22I KspAI KspI MbiI MfeI MflI MhlI MlsI
MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI MroNI
MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI NsbI
NsiI NspI NspV PaeI PaeR7I PagI PauI PceI
PciI PctI PdiI Pfl23II PinAI Ple19I PluTI PmaCI
PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I PspCI
PspFI PspLI PspN4I PspOMI PstI PsuI PteI PvuI
PvuII RruI SacI SacII SalI ScaI SduI SfcI
SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI SmlI
SmoI SnaBI SpeI SphI SseBI SspDI SspI SstI
StuI StyI TaqII TatI TspMI Vha464I VneI VspI
XapI XbaI XceI XhoI XmaI XmaJI XmiI ZraI
ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I Bpu1102I
Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI CsiI
CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI MabI
PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI Rsr2I
RsrII SapI SexAI AbsI AscI AsiSI CciNI FseI
FspAI MauBI MreI MssI NotI PacI PalAI PmeI
PspXI RgaI RigI SbfI SdaI SfaAI SgfI SgrAI
SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI AlwNI
CaiI DraIII MteI PflFI PstNI PsyI Tth111I AleI
Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI MslI
OliI PdmI PshAI RseI SmiMI XmnI AccB7I AfiI
AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I BseLI
BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI FalI
HpyF10VI MwoI PflMI Van91I XagI AasI BcgI BstXI
CspCI DrdI DseDI AloI ArsI BarI PcsI PsrI
SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, HpyCH4III, FaeI, FatI, Bst4CI, TaaI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 AbaSI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
ATACGATGTCATGCG
|||||||||||||||
TATGCTACAGTACGC
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I
BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI
DdeI Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI
GluI HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III
HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI MspR9I
MvaI NciI NmuCI PfeI PkrI PleI PpsI Psp6I
PspGI PspPI SatI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI TseI Tsp45I
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspA1I MspCI MunI
Mva1269I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV
NmeAIII NruI NsbI NsiI NspI NspV PaeI PaeR7I
PagI PauI PceI PciI PctI PdiI Pfl23II PinAI
Ple19I PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI
Psp124BI Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI
PsuI PteI PvuI PvuII RruI SacI SacII SalI
ScaI SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI
SlaI SmaI SmlI SmoI SnaBI SpeI SphI SseBI
SspDI SspI SstI StuI StyI TaqII TatI TspMI
Vha464I VneI VspI XapI XbaI XceI XhoI XmaI
XmaJI XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI
BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI
Bsu36I CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I
KflI LguI MabI PasI PciSI PfoI PpuMI Psp5II
PspEI PspPPI Rsr2I RsrII SapI SexAI AbsI AscI
AsiSI CciNI FseI FspAI MauBI MreI MssI NotI
PacI PalAI PmeI PspXI RgaI RigI SbfI SdaI
SfaAI SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I
SwaI AdeI AlwNI CaiI DraIII MteI PflFI PstNI
PsyI Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI
BstPAI MroXI MslI OliI PdmI PshAI RseI SmiMI
XmnI AccB7I AfiI AhdI BaeI BglI BmeRI BplI
BsaXI Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI
Eam1105I EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI
AasI BcgI BstXI CspCI DrdI DseDI AloI ArsI
BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 MaeII AbaSI HpyCH4IV HpySE526I
|
| 7 TaiI SetI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
ATACGTTGTCATGCT
|||||||||||||||
TATGCAACAGTACGA
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4V HspAI Kzo9I LpnPI MaeI MalI MboI MluCI
MnlI MseI MspI MspJI MvnI NdeII RsaI RsaNI
SaqAI Sau3AI SgeI Sse9I SsiI SspMI TaqI TasI
Tru1I Tru9I XspI AclWI AgsI AjnI Alw26I AlwI
ApeKI AspS9I AsuC2I AsuHPI AvaII BbvI BccI BceAI
BciT130I BcnI BcoDI BisI BlsI Bme1390I Bme18I BmgT120I
BmrFI BmsI BpuMI Bse1I BseBI BseGI BseMII BseNI
BseXI BslFI BsmAI BsmFI BspCNI BspPI BsrI Bst2UI
Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI BstV1I BtsCI
BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII FaqI FauI
Fnu4HI FokI Fsp4HI GluI HgaI HinfI HphI Hpy188I
Hpy99I HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII
MboII MlyI MspR9I MvaI NciI NmuCI PfeI PkrI
PleI PpsI Psp6I PspGI PspPI SatI Sau96I SchI
ScrFI SfaNI SinI StyD4I TaaI TauI TfiI TscAI
TseFI TseI Tsp45I TspDTI TspGWI TspRI VpaK11BI AanI
AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanI
BanII BauI BbrPI BbsI Bbv12I BciVI BclI BcuI
BfmI BfoI BfrI BfuAI BfuI BglII BlnI BmcAI
BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI BpmI
Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI
Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI
BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI
BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI
Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI
BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I
BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI
BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I
BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I
BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI
BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI
BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I
ClaI CspAI DinI DraI EaeI EagI Eam1104I EarI
EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I
Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI
EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI
Esp3I FauNDI FbaI FblI FriOI FspI GsaI GsuI
HaeII Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III
Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI
KspI MbiI MfeI MflI MhlI MlsI MluI MluNI
Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I
MspA1I MspCI MunI Mva1269I NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI NspI
NspV PaeI PaeR7I PagI PauI PceI PciI PctI
PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I
PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI
PspN4I PspOMI PstI PsuI PteI PvuI PvuII RruI
SacI SacII SalI ScaI SduI SfcI SfoI Sfr274I
Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI
SpeI SphI SseBI SspDI SspI SstI StuI StyI
TaqII TatI TspMI Vha464I VneI VspI XapI XbaI
XceI XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I
AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I
BspQI BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I
Eco91I EcoO109I EcoO65I KflI LguI MabI PasI PciSI
PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI
SexAI AbsI AscI AsiSI CciNI FseI FspAI MauBI
MreI MssI NotI PacI PalAI PmeI PspXI RgaI
RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI
SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII
MteI PflFI PstNI PsyI Tth111I AleI Asp700I BoxI
BsaBI Bse8I BseJI BstPAI MroXI MslI OliI PdmI
PshAI RseI SmiMI XmnI AccB7I AfiI AhdI BaeI
BglI BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI
BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI
PflMI Van91I XagI AasI BcgI BstXI CspCI DrdI
DseDI AloI ArsI BarI PcsI PsrI SfiI AjuI
XcmI
Proposed sequence enzymes: MaeII, NlaIII, AbaSI, CviAII, HpyCH4IV, TaiI, FaeI, HpySE526I, FaiI, FatI, Hsp92II, SetI, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 8 TseFI NmuCI Tsp45I MaeIII
| |
ATTCGCTGTCACGCA
|||||||||||||||
TAAGCGACAGTGCGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviAII CviJI CviKI_1 CviQI DpnI
DpnII FaeI FaiI FatI FspBI GlaI HaeIII HapII
HhaI Hin1II Hin6I HinP1I HpaII HpyCH4IV HpyCH4V HpySE526I
Hsp92II HspAI Kzo9I LpnPI MaeI MaeII MalI MboI
MluCI MnlI MseI MspI MspJI MvnI NdeII NlaIII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI BisI
BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I
BseBI BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI
BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI
BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI
Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI
HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I
LmnI Lsp1109I LweI MboII MlyI MspR9I MvaI NciI
PfeI PkrI PleI PpsI Psp6I PspGI PspPI SatI
Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI TauI
TfiI TscAI TseI TspDTI TspGWI TspRI VpaK11BI AanI
AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanI
BanII BauI BbrPI BbsI Bbv12I BciVI BclI BcuI
BfmI BfoI BfrI BfuAI BfuI BglII BlnI BmcAI
BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI BpmI
Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI
Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI
BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI
BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI
Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI
BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I
BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI
BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I
BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I
BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI
BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI
BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I
ClaI CspAI DinI DraI EaeI EagI Eam1104I EarI
EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I
Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI
EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI
Esp3I FauNDI FbaI FblI FriOI FspI GsaI GsuI
HaeII Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III
Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI
KspI MbiI MfeI MflI MhlI MlsI MluI MluNI
Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I
MspA1I MspCI MunI Mva1269I NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI NspI
NspV PaeI PaeR7I PagI PauI PceI PciI PctI
PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I
PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI
PspN4I PspOMI PstI PsuI PteI PvuI PvuII RruI
SacI SacII SalI ScaI SduI SfcI SfoI Sfr274I
Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI
SpeI SphI SseBI SspDI SspI SstI StuI StyI
TaqII TatI TspMI Vha464I VneI VspI XapI XbaI
XceI XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I
AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I
BspQI BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I
Eco91I EcoO109I EcoO65I KflI LguI MabI PasI PciSI
PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI
SexAI AbsI AscI AsiSI CciNI FseI FspAI MauBI
MreI MssI NotI PacI PalAI PmeI PspXI RgaI
RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI
SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII
MteI PflFI PstNI PsyI Tth111I AleI Asp700I BoxI
BsaBI Bse8I BseJI BstPAI MroXI MslI OliI PdmI
PshAI RseI SmiMI XmnI AccB7I AfiI AhdI BaeI
BglI BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI
BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI
PflMI Van91I XagI AasI BcgI BstXI CspCI DrdI
DseDI AloI ArsI BarI PcsI PsrI SfiI AjuI
XcmI
Proposed sequence enzymes: TseFI, AbaSI, MaeIII, Tsp45I, NmuCI
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 10 FatI
| |
| |11 CviAII
| ||
| ||12 FaiI
| |||
| ||| 14 Hin1II NlaIII FaeI Hsp92II
| ||| |
ATTCGCTGTCATGCT
|||||||||||||||
TAAGCGACAGTACGA
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I
BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI
DdeI Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI
GluI HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III
HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI MspR9I
MvaI NciI NmuCI PfeI PkrI PleI PpsI Psp6I
PspGI PspPI SatI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI TseI Tsp45I
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspA1I MspCI MunI
Mva1269I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV
NmeAIII NruI NsbI NsiI NspI NspV PaeI PaeR7I
PagI PauI PceI PciI PctI PdiI Pfl23II PinAI
Ple19I PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI
Psp124BI Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI
PsuI PteI PvuI PvuII RruI SacI SacII SalI
ScaI SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI
SlaI SmaI SmlI SmoI SnaBI SpeI SphI SseBI
SspDI SspI SstI StuI StyI TaqII TatI TspMI
Vha464I VneI VspI XapI XbaI XceI XhoI XmaI
XmaJI XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI
BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI
Bsu36I CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I
KflI LguI MabI PasI PciSI PfoI PpuMI Psp5II
PspEI PspPPI Rsr2I RsrII SapI SexAI AbsI AscI
AsiSI CciNI FseI FspAI MauBI MreI MssI NotI
PacI PalAI PmeI PspXI RgaI RigI SbfI SdaI
SfaAI SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I
SwaI AdeI AlwNI CaiI DraIII MteI PflFI PstNI
PsyI Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI
BstPAI MroXI MslI OliI PdmI PshAI RseI SmiMI
XmnI AccB7I AfiI AhdI BaeI BglI BmeRI BplI
BsaXI Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI
Eam1105I EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI
AasI BcgI BstXI CspCI DrdI DseDI AloI ArsI
BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
3 BsaWI
|
|4 MspI BsiSI AbaSI HapII HpaII
||
|| 10 FatI
|| |
|| |11 CviAII
|| ||
|| ||12 FaiI
|| |||
|| ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
|| ||| |
|| ||| |15 AbaSI
|| ||| ||
ATCCGGTGTCATGCA
|||||||||||||||
TAGGCCACAGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsnI Bsp143I BspACI BspANI
BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI BsuRI
CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII FspBI
GlaI HaeIII HhaI Hin6I HinP1I HpyCH4IV HpySE526I HspAI
Kzo9I LpnPI MaeI MaeII MalI MboI MluCI MnlI
MseI MspJI MvnI NdeII RsaI RsaNI SaqAI Sau3AI
SetI SgeI Sse9I SsiI SspMI TaiI TaqI TasI
Tru1I Tru9I XspI AclWI AgsI AjnI Alw26I AlwI
ApeKI AspS9I AsuC2I AsuHPI AvaII BbvI BccI BceAI
BciT130I BcnI BcoDI BisI BlsI Bme1390I Bme18I BmgT120I
BmrFI BmsI BpuMI Bse1I BseBI BseGI BseMII BseNI
BseXI BslFI BsmAI BsmFI BspCNI BspPI BsrI Bst2UI
Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI BstV1I BtsCI
BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII FaqI FauI
Fnu4HI FokI Fsp4HI GluI HgaI HinfI HphI Hpy188I
Hpy99I HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII
MboII MlyI MspR9I MvaI NciI NmuCI PfeI PkrI
PleI PpsI Psp6I PspGI PspPI SatI Sau96I SchI
ScrFI SfaNI SinI StyD4I TaaI TauI TfiI TscAI
TseFI TseI Tsp45I TspDTI TspGWI TspRI VpaK11BI AanI
AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanI
BanII BauI BbrPI BbsI Bbv12I BciVI BclI BcuI
BfmI BfoI BfrI BfuAI BfuI BglII BlnI BmcAI
BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI BpmI
Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI Bse118I
Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI BseSI
BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI BsiEI
BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I
Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI
BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I BspTI
BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI BssHII
BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI
BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I BstMCI
BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I
BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI BtsI
BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI
CspAI DinI DraI EaeI EagI Eam1104I EarI EciI
Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I
Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI
EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI Esp3I
FauNDI FbaI FblI FriOI FspI GsaI GsuI HaeII
Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III Hpy8I
Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI KspI
MbiI MfeI MflI MhlI MlsI MluI MluNI Mly113I
MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I MspA1I
MspCI MunI Mva1269I NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NmeAIII NruI NsbI NsiI NspI NspV
PaeI PaeR7I PagI PauI PceI PciI PctI PdiI
Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I PscI
PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI PspN4I
PspOMI PstI PsuI PteI PvuI PvuII RruI SacI
SacII SalI ScaI SduI SfcI SfoI Sfr274I Sfr303I
SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI SpeI
SphI SseBI SspDI SspI SstI StuI StyI TaqII
TatI TspMI Vha464I VneI VspI XapI XbaI XceI
XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I AarI
AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI
BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I Eco91I
EcoO109I EcoO65I KflI LguI MabI PasI PciSI PfoI
PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI SexAI
AbsI AscI AsiSI CciNI FseI FspAI MauBI MreI
MssI NotI PacI PalAI PmeI PspXI RgaI RigI
SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI SmiI
SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII MteI
PflFI PstNI PsyI Tth111I AleI Asp700I BoxI BsaBI
Bse8I BseJI BstPAI MroXI MslI OliI PdmI PshAI
RseI SmiMI XmnI AccB7I AfiI AhdI BaeI BglI
BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI BstENI
BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI PflMI
Van91I XagI AasI BcgI BstXI CspCI DrdI DseDI
AloI ArsI BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: BsaWI, MspI, AbaSI, CviAII, BsiSI, HapII, FaeI, FaiI, HpaII, FatI, HpyCH4V, Hsp92II, NlaIII, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 8 TseFI NmuCI Tsp45I MaeIII
| |
ATACGCTGTCACGCT
|||||||||||||||
TATGCGACAGTGCGA
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviAII CviJI CviKI_1 CviQI DpnI
DpnII FaeI FaiI FatI FspBI GlaI HaeIII HapII
HhaI Hin1II Hin6I HinP1I HpaII HpyCH4IV HpyCH4V HpySE526I
Hsp92II HspAI Kzo9I LpnPI MaeI MaeII MalI MboI
MluCI MnlI MseI MspI MspJI MvnI NdeII NlaIII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI BisI
BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I
BseBI BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI
BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI
BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI
Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI
HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I
LmnI Lsp1109I LweI MboII MlyI MspR9I MvaI NciI
PfeI PkrI PleI PpsI Psp6I PspGI PspPI SatI
Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI TauI
TfiI TscAI TseI TspDTI TspGWI TspRI VpaK11BI AanI
AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanI
BanII BauI BbrPI BbsI Bbv12I BciVI BclI BcuI
BfmI BfoI BfrI BfuAI BfuI BglII BlnI BmcAI
BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI BpmI
Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI
Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI
BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI
BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI
Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI
BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I
BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI
BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I
BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I
BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI
BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI
BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I
ClaI CspAI DinI DraI EaeI EagI Eam1104I EarI
EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I
Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI
EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI
Esp3I FauNDI FbaI FblI FriOI FspI GsaI GsuI
HaeII Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III
Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI
KspI MbiI MfeI MflI MhlI MlsI MluI MluNI
Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I
MspA1I MspCI MunI Mva1269I NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI NspI
NspV PaeI PaeR7I PagI PauI PceI PciI PctI
PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I
PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI
PspN4I PspOMI PstI PsuI PteI PvuI PvuII RruI
SacI SacII SalI ScaI SduI SfcI SfoI Sfr274I
Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI
SpeI SphI SseBI SspDI SspI SstI StuI StyI
TaqII TatI TspMI Vha464I VneI VspI XapI XbaI
XceI XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I
AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I
BspQI BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I
Eco91I EcoO109I EcoO65I KflI LguI MabI PasI PciSI
PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI
SexAI AbsI AscI AsiSI CciNI FseI FspAI MauBI
MreI MssI NotI PacI PalAI PmeI PspXI RgaI
RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI
SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII
MteI PflFI PstNI PsyI Tth111I AleI Asp700I BoxI
BsaBI Bse8I BseJI BstPAI MroXI MslI OliI PdmI
PshAI RseI SmiMI XmnI AccB7I AfiI AhdI BaeI
BglI BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI
BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI
PflMI Van91I XagI AasI BcgI BstXI CspCI DrdI
DseDI AloI ArsI BarI PcsI PsrI SfiI AjuI
XcmI
Proposed sequence enzymes: TseFI, AbaSI, MaeIII, Tsp45I, NmuCI
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 AbaSI HpyCH4III Bst4CI TaaI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
ATACGGTGTCATGCG
|||||||||||||||
TATGCCACAGTACGC
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI BstDEI BstF5I BstMAI
BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI
Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI
HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyF3I LmnI
Lsp1109I LweI MaeIII MboII MlyI MspR9I MvaI NciI
NmuCI PfeI PkrI PleI PpsI Psp6I PspGI PspPI
SatI Sau96I SchI ScrFI SfaNI SinI StyD4I TauI
TfiI TscAI TseFI TseI Tsp45I TspDTI TspGWI TspRI
VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I AccBSI
AccI AccIII AclI AcoI AcsI AcuI AcvI AcyI
AfeI AflII AflIII AgeI AhlI AjiI Alw21I Alw44I
Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI AsiGI
Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI BalI
BamHI BanI BanII BauI BbrPI BbsI Bbv12I BciVI
BclI BcuI BfmI BfoI BfrI BfuAI BfuI BglII
BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI BmuI
BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI
BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI BseMI
BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I BshNI
BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI
Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I
Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI BspOI
BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI BsrGI
BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I Bst1107I
Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI BstC8I
BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I
BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI
BtgZI BtrI BtsI BtuMI BveI Cac8I CciI Cfr10I
Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI EagI
Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I Eco147I
Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I
Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI
EheI ErhI Esp3I FauNDI FbaI FblI FriOI FspI
GsaI GsuI HaeII Hin1I HincII HindII HindIII HpaI
Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI KroI
Ksp22I KspAI KspI MbiI MfeI MflI MhlI MlsI
MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI MroNI
MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI NsbI
NsiI NspI NspV PaeI PaeR7I PagI PauI PceI
PciI PctI PdiI Pfl23II PinAI Ple19I PluTI PmaCI
PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I PspCI
PspFI PspLI PspN4I PspOMI PstI PsuI PteI PvuI
PvuII RruI SacI SacII SalI ScaI SduI SfcI
SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI SmlI
SmoI SnaBI SpeI SphI SseBI SspDI SspI SstI
StuI StyI TaqII TatI TspMI Vha464I VneI VspI
XapI XbaI XceI XhoI XmaI XmaJI XmiI ZraI
ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I Bpu1102I
Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI CsiI
CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI MabI
PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI Rsr2I
RsrII SapI SexAI AbsI AscI AsiSI CciNI FseI
FspAI MauBI MreI MssI NotI PacI PalAI PmeI
PspXI RgaI RigI SbfI SdaI SfaAI SgfI SgrAI
SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI AlwNI
CaiI DraIII MteI PflFI PstNI PsyI Tth111I AleI
Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI MslI
OliI PdmI PshAI RseI SmiMI XmnI AccB7I AfiI
AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I BseLI
BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI FalI
HpyF10VI MwoI PflMI Van91I XagI AasI BcgI BstXI
CspCI DrdI DseDI AloI ArsI BarI PcsI PsrI
SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, HpyCH4III, FaeI, FatI, Bst4CI, TaaI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 MaeII AbaSI HpyCH4IV HpySE526I
|
| 7 TaiI SetI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
ATACGTTGTCATGCC
|||||||||||||||
TATGCAACAGTACGG
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4V HspAI Kzo9I LpnPI MaeI MalI MboI MluCI
MnlI MseI MspI MspJI MvnI NdeII RsaI RsaNI
SaqAI Sau3AI SgeI Sse9I SsiI SspMI TaqI TasI
Tru1I Tru9I XspI AclWI AgsI AjnI Alw26I AlwI
ApeKI AspS9I AsuC2I AsuHPI AvaII BbvI BccI BceAI
BciT130I BcnI BcoDI BisI BlsI Bme1390I Bme18I BmgT120I
BmrFI BmsI BpuMI Bse1I BseBI BseGI BseMII BseNI
BseXI BslFI BsmAI BsmFI BspCNI BspPI BsrI Bst2UI
Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI BstV1I BtsCI
BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII FaqI FauI
Fnu4HI FokI Fsp4HI GluI HgaI HinfI HphI Hpy188I
Hpy99I HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII
MboII MlyI MspR9I MvaI NciI NmuCI PfeI PkrI
PleI PpsI Psp6I PspGI PspPI SatI Sau96I SchI
ScrFI SfaNI SinI StyD4I TaaI TauI TfiI TscAI
TseFI TseI Tsp45I TspDTI TspGWI TspRI VpaK11BI AanI
AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanI
BanII BauI BbrPI BbsI Bbv12I BciVI BclI BcuI
BfmI BfoI BfrI BfuAI BfuI BglII BlnI BmcAI
BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI BpmI
Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI
Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI
BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI
BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI
Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI
BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I
BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI
BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I
BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I
BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI
BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI
BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I
ClaI CspAI DinI DraI EaeI EagI Eam1104I EarI
EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I
Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI
EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI
Esp3I FauNDI FbaI FblI FriOI FspI GsaI GsuI
HaeII Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III
Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI
KspI MbiI MfeI MflI MhlI MlsI MluI MluNI
Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I
MspA1I MspCI MunI Mva1269I NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI NspI
NspV PaeI PaeR7I PagI PauI PceI PciI PctI
PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I
PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI
PspN4I PspOMI PstI PsuI PteI PvuI PvuII RruI
SacI SacII SalI ScaI SduI SfcI SfoI Sfr274I
Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI
SpeI SphI SseBI SspDI SspI SstI StuI StyI
TaqII TatI TspMI Vha464I VneI VspI XapI XbaI
XceI XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I
AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I
BspQI BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I
Eco91I EcoO109I EcoO65I KflI LguI MabI PasI PciSI
PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI
SexAI AbsI AscI AsiSI CciNI FseI FspAI MauBI
MreI MssI NotI PacI PalAI PmeI PspXI RgaI
RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI
SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII
MteI PflFI PstNI PsyI Tth111I AleI Asp700I BoxI
BsaBI Bse8I BseJI BstPAI MroXI MslI OliI PdmI
PshAI RseI SmiMI XmnI AccB7I AfiI AhdI BaeI
BglI BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI
BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI
PflMI Van91I XagI AasI BcgI BstXI CspCI DrdI
DseDI AloI ArsI BarI PcsI PsrI SfiI AjuI
XcmI
Proposed sequence enzymes: MaeII, NlaIII, AbaSI, CviAII, HpyCH4IV, TaiI, FaeI, HpySE526I, FaiI, FatI, Hsp92II, SetI, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 HpyCH4III Bst4CI TaaI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
ATACGGTGTCATGCT
|||||||||||||||
TATGCCACAGTACGA
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI BstDEI BstF5I BstMAI
BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI
Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI
HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyF3I LmnI
Lsp1109I LweI MaeIII MboII MlyI MspR9I MvaI NciI
NmuCI PfeI PkrI PleI PpsI Psp6I PspGI PspPI
SatI Sau96I SchI ScrFI SfaNI SinI StyD4I TauI
TfiI TscAI TseFI TseI Tsp45I TspDTI TspGWI TspRI
VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I AccBSI
AccI AccIII AclI AcoI AcsI AcuI AcvI AcyI
AfeI AflII AflIII AgeI AhlI AjiI Alw21I Alw44I
Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI AsiGI
Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI BalI
BamHI BanI BanII BauI BbrPI BbsI Bbv12I BciVI
BclI BcuI BfmI BfoI BfrI BfuAI BfuI BglII
BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI BmuI
BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI
BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI BseMI
BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I BshNI
BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI
Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I
Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI BspOI
BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI BsrGI
BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I Bst1107I
Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI BstC8I
BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I
BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI
BtgZI BtrI BtsI BtuMI BveI Cac8I CciI Cfr10I
Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI EagI
Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I Eco147I
Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I
Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI
EheI ErhI Esp3I FauNDI FbaI FblI FriOI FspI
GsaI GsuI HaeII Hin1I HincII HindII HindIII HpaI
Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI KroI
Ksp22I KspAI KspI MbiI MfeI MflI MhlI MlsI
MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI MroNI
MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI NarI
NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI NsbI
NsiI NspI NspV PaeI PaeR7I PagI PauI PceI
PciI PctI PdiI Pfl23II PinAI Ple19I PluTI PmaCI
PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I PspCI
PspFI PspLI PspN4I PspOMI PstI PsuI PteI PvuI
PvuII RruI SacI SacII SalI ScaI SduI SfcI
SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI SmlI
SmoI SnaBI SpeI SphI SseBI SspDI SspI SstI
StuI StyI TaqII TatI TspMI Vha464I VneI VspI
XapI XbaI XceI XhoI XmaI XmaJI XmiI ZraI
ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I Bpu1102I
Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI CsiI
CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI MabI
PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI Rsr2I
RsrII SapI SexAI AbsI AscI AsiSI CciNI FseI
FspAI MauBI MreI MssI NotI PacI PalAI PmeI
PspXI RgaI RigI SbfI SdaI SfaAI SgfI SgrAI
SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI AlwNI
CaiI DraIII MteI PflFI PstNI PsyI Tth111I AleI
Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI MslI
OliI PdmI PshAI RseI SmiMI XmnI AccB7I AfiI
AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I BseLI
BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI FalI
HpyF10VI MwoI PflMI Van91I XagI AasI BcgI BstXI
CspCI DrdI DseDI AloI ArsI BarI PcsI PsrI
SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, HpyCH4III, FaeI, FatI, Bst4CI, TaaI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 8 TseFI NmuCI Tsp45I MaeIII
| |
ATACGATGTCACGCA
|||||||||||||||
TATGCTACAGTGCGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviAII CviJI CviKI_1 CviQI DpnI
DpnII FaeI FaiI FatI FspBI GlaI HaeIII HapII
HhaI Hin1II Hin6I HinP1I HpaII HpyCH4IV HpyCH4V HpySE526I
Hsp92II HspAI Kzo9I LpnPI MaeI MaeII MalI MboI
MluCI MnlI MseI MspI MspJI MvnI NdeII NlaIII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI BisI
BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I
BseBI BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI
BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI
BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI
Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI
HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I
LmnI Lsp1109I LweI MboII MlyI MspR9I MvaI NciI
PfeI PkrI PleI PpsI Psp6I PspGI PspPI SatI
Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI TauI
TfiI TscAI TseI TspDTI TspGWI TspRI VpaK11BI AanI
AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanI
BanII BauI BbrPI BbsI Bbv12I BciVI BclI BcuI
BfmI BfoI BfrI BfuAI BfuI BglII BlnI BmcAI
BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI BpmI
Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI
Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI
BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI
BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI
Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI
BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I
BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI
BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I
BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I
BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI
BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI
BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I
ClaI CspAI DinI DraI EaeI EagI Eam1104I EarI
EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I
Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI
EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI
Esp3I FauNDI FbaI FblI FriOI FspI GsaI GsuI
HaeII Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III
Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI
KspI MbiI MfeI MflI MhlI MlsI MluI MluNI
Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I
MspA1I MspCI MunI Mva1269I NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI NspI
NspV PaeI PaeR7I PagI PauI PceI PciI PctI
PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I
PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI
PspN4I PspOMI PstI PsuI PteI PvuI PvuII RruI
SacI SacII SalI ScaI SduI SfcI SfoI Sfr274I
Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI
SpeI SphI SseBI SspDI SspI SstI StuI StyI
TaqII TatI TspMI Vha464I VneI VspI XapI XbaI
XceI XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I
AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I
BspQI BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I
Eco91I EcoO109I EcoO65I KflI LguI MabI PasI PciSI
PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI
SexAI AbsI AscI AsiSI CciNI FseI FspAI MauBI
MreI MssI NotI PacI PalAI PmeI PspXI RgaI
RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI
SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII
MteI PflFI PstNI PsyI Tth111I AleI Asp700I BoxI
BsaBI Bse8I BseJI BstPAI MroXI MslI OliI PdmI
PshAI RseI SmiMI XmnI AccB7I AfiI AhdI BaeI
BglI BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI
BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI
PflMI Van91I XagI AasI BcgI BstXI CspCI DrdI
DseDI AloI ArsI BarI PcsI PsrI SfiI AjuI
XcmI
Proposed sequence enzymes: TseFI, AbaSI, MaeIII, Tsp45I, NmuCI
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 HpyCH4III Bst4CI TaaI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| | ||| |
ATACGGTGTCATGCA
|||||||||||||||
TATGCCACAGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI
MboI MluCI MnlI MseI MspI MspJI MvnI NdeII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI BisI
BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I
BseBI BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI
BspCNI BspPI BsrI Bst2UI BstDEI BstF5I BstMAI BstNI
BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I
EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI HgaI
HinfI HphI Hpy188I Hpy99I HpyAV HpyF3I LmnI Lsp1109I
LweI MaeIII MboII MlyI MspR9I MvaI NciI NmuCI
PfeI PkrI PleI PpsI Psp6I PspGI PspPI SatI
Sau96I SchI ScrFI SfaNI SinI StyD4I TauI TfiI
TscAI TseFI TseI Tsp45I TspDTI TspGWI TspRI VpaK11BI
AanI AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI
AccIII AclI AcoI AcsI AcuI AcvI AcyI AfeI
AflII AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I
Aor13HI Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I
AspA2I AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI
BanI BanII BauI BbrPI BbsI Bbv12I BciVI BclI
BcuI BfmI BfoI BfrI BfuAI BfuI BglII BlnI
BmcAI BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI
BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI
BsaWI Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI
BseRI BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI
BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I
BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I
BspDI BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I
BspT107I BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI
BssECI BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI
Bst6I BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI
BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I
BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI
BtrI BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I
Cfr9I ClaI CspAI DinI DraI EaeI EagI Eam1104I
EarI EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I
Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I
EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI
ErhI Esp3I FauNDI FbaI FblI FriOI FspI GsaI
GsuI HaeII Hin1I HincII HindII HindIII HpaI Hpy166II
Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I
KspAI KspI MbiI MfeI MflI MhlI MlsI MluI
MluNI Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI
Msp20I MspA1I MspCI MunI Mva1269I NaeI NarI NcoI
NdeI NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI
NspI NspV PaeI PaeR7I PagI PauI PceI PciI
PctI PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI
Ppu21I PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI
PspLI PspN4I PspOMI PstI PsuI PteI PvuI PvuII
RruI SacI SacII SalI ScaI SduI SfcI SfoI
Sfr274I Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI
SnaBI SpeI SphI SseBI SspDI SspI SstI StuI
StyI TaqII TatI TspMI Vha464I VneI VspI XapI
XbaI XceI XhoI XmaI XmaJI XmiI ZraI ZrmI
Zsp2I AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I
Bsp1720I BspQI BstEII BstPI Bsu36I CpoI CsiI CspI
Eco81I Eco91I EcoO109I EcoO65I KflI LguI MabI PasI
PciSI PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII
SapI SexAI AbsI AscI AsiSI CciNI FseI FspAI
MauBI MreI MssI NotI PacI PalAI PmeI PspXI
RgaI RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI
SgsI SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI
DraIII MteI PflFI PstNI PsyI Tth111I AleI Asp700I
BoxI BsaBI Bse8I BseJI BstPAI MroXI MslI OliI
PdmI PshAI RseI SmiMI XmnI AccB7I AfiI AhdI
BaeI BglI BmeRI BplI BsaXI Bsc4I BseLI BslI
BstAPI BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI
MwoI PflMI Van91I XagI AasI BcgI BstXI CspCI
DrdI DseDI AloI ArsI BarI PcsI PsrI SfiI
AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, HpyCH4III, FaeI, FatI, HpyCH4V, Bst4CI, TaaI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 8 TseFI NmuCI Tsp45I MaeIII
| |
ATACGCTGTCACGCA
|||||||||||||||
TATGCGACAGTGCGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviAII CviJI CviKI_1 CviQI DpnI
DpnII FaeI FaiI FatI FspBI GlaI HaeIII HapII
HhaI Hin1II Hin6I HinP1I HpaII HpyCH4IV HpyCH4V HpySE526I
Hsp92II HspAI Kzo9I LpnPI MaeI MaeII MalI MboI
MluCI MnlI MseI MspI MspJI MvnI NdeII NlaIII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI BisI
BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I
BseBI BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI
BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI
BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI
Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI
HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I
LmnI Lsp1109I LweI MboII MlyI MspR9I MvaI NciI
PfeI PkrI PleI PpsI Psp6I PspGI PspPI SatI
Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI TauI
TfiI TscAI TseI TspDTI TspGWI TspRI VpaK11BI AanI
AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanI
BanII BauI BbrPI BbsI Bbv12I BciVI BclI BcuI
BfmI BfoI BfrI BfuAI BfuI BglII BlnI BmcAI
BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI BpmI
Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI
Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI
BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI
BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI
Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI
BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I
BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI
BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I
BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I
BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI
BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI
BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I
ClaI CspAI DinI DraI EaeI EagI Eam1104I EarI
EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I
Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI
EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI
Esp3I FauNDI FbaI FblI FriOI FspI GsaI GsuI
HaeII Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III
Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI
KspI MbiI MfeI MflI MhlI MlsI MluI MluNI
Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I
MspA1I MspCI MunI Mva1269I NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI NspI
NspV PaeI PaeR7I PagI PauI PceI PciI PctI
PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I
PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI
PspN4I PspOMI PstI PsuI PteI PvuI PvuII RruI
SacI SacII SalI ScaI SduI SfcI SfoI Sfr274I
Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI
SpeI SphI SseBI SspDI SspI SstI StuI StyI
TaqII TatI TspMI Vha464I VneI VspI XapI XbaI
XceI XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I
AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I
BspQI BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I
Eco91I EcoO109I EcoO65I KflI LguI MabI PasI PciSI
PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI
SexAI AbsI AscI AsiSI CciNI FseI FspAI MauBI
MreI MssI NotI PacI PalAI PmeI PspXI RgaI
RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI
SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII
MteI PflFI PstNI PsyI Tth111I AleI Asp700I BoxI
BsaBI Bse8I BseJI BstPAI MroXI MslI OliI PdmI
PshAI RseI SmiMI XmnI AccB7I AfiI AhdI BaeI
BglI BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI
BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI
PflMI Van91I XagI AasI BcgI BstXI CspCI DrdI
DseDI AloI ArsI BarI PcsI PsrI SfiI AjuI
XcmI
Proposed sequence enzymes: TseFI, AbaSI, MaeIII, Tsp45I, NmuCI
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 ApeKI TseI
| |
| |7 GluI Fsp4HI Fnu4HI SatI BisI
| ||
| ||8 PkrI BlsI
| |||
| ||| 10 FatI
| ||| |
| ||| |11 CviAII
| ||| ||
| ||| ||12 FaiI
| ||| |||
| ||| ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| ||| ||| |
ATTCGCTGCCATGCA
|||||||||||||||
TAAGCGACGGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI
MboI MluCI MnlI MseI MspI MspJI MvnI NdeII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI AspS9I AsuC2I AsuHPI AvaII
BbvI BccI BceAI BciT130I BcnI BcoDI Bme1390I Bme18I
BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI BseMII
BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI BsrI
Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI BstV1I
BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII FaqI
FauI FokI HgaI HinfI HphI Hpy188I Hpy99I HpyAV
HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI
MspR9I MvaI NciI NmuCI PfeI PleI PpsI Psp6I
PspGI PspPI Sau96I SchI ScrFI SfaNI SinI StyD4I
TaaI TauI TfiI TscAI TseFI Tsp45I TspDTI TspGWI
TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I
AccBSI AccI AccIII AclI AcoI AcsI AcuI AcvI
AcyI AfeI AflII AflIII AgeI AhlI AjiI Alw21I
Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI
AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI
BalI BamHI BanI BanII BauI BbrPI BbsI Bbv12I
BciVI BclI BcuI BfmI BfoI BfrI BfuAI BfuI
BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI
BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI
BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI
BseMI BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I
BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI
BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I
Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI
BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI
BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I
Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI
BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI
BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI
BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I CciI
Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI
EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I
Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I
Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I
EgeI EheI ErhI Esp3I FauNDI FbaI FblI FriOI
FspI GsaI GsuI HaeII Hin1I HincII HindII HindIII
HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI
KroI Ksp22I KspAI KspI MbiI MfeI MflI MhlI
MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI
MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI
NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI
NsbI NsiI NspI NspV PaeI PaeR7I PagI PauI
PceI PciI PctI PdiI Pfl23II PinAI Ple19I PluTI
PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I
PspCI PspFI PspLI PspN4I PspOMI PstI PsuI PteI
PvuI PvuII RruI SacI SacII SalI ScaI SduI
SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI
SmlI SmoI SnaBI SpeI SphI SseBI SspDI SspI
SstI StuI StyI TaqII TatI TspMI Vha464I VneI
VspI XapI XbaI XceI XhoI XmaI XmaJI XmiI
ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I
Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI
CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI
MabI PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI
Rsr2I RsrII SapI SexAI AbsI AscI AsiSI CciNI
FseI FspAI MauBI MreI MssI NotI PacI PalAI
PmeI PspXI RgaI RigI SbfI SdaI SfaAI SgfI
SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI
AlwNI CaiI DraIII MteI PflFI PstNI PsyI Tth111I
AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI
MslI OliI PdmI PshAI RseI SmiMI XmnI AccB7I
AfiI AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I
BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI
FalI HpyF10VI MwoI PflMI Van91I XagI AasI BcgI
BstXI CspCI DrdI DseDI AloI ArsI BarI PcsI
PsrI SfiI AjuI XcmI
Proposed sequence enzymes: TseI, SatI, GluI, AbaSI, Fsp4HI, CviAII, FaiI, FaeI, FatI, HpyCH4V, Fnu4HI, PkrI, Hsp92II, ApeKI, BisI, BlsI, NlaIII, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AciI AbaSI BspACI SsiI
|
| 6 MspA1I ApeKI TseI
| |
| |7 GluI Fsp4HI Fnu4HI SatI BisI
| ||
| ||8 PkrI BlsI
| |||
| ||| 10 FatI
| ||| |
| ||| |11 CviAII
| ||| ||
| ||| ||12 FaiI
| ||| |||
| ||| ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| ||| ||| |
| ||| ||| |15 AbaSI
| ||| ||| ||
ATCCGCTGCCATGCA
|||||||||||||||
TAGGCGACGGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AfaI AluBI AluI AoxI AspLEI BfaI
BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspANI BspFNI
BssMI BstFNI BstHHI BstKTI BstMBI BstUI BsuRI CfoI
Csp6I CviJI CviKI_1 CviQI DpnI DpnII FspBI GlaI
HaeIII HapII HhaI Hin6I HinP1I HpaII HpyCH4IV HpySE526I
HspAI Kzo9I LpnPI MaeI MaeII MalI MboI MluCI
MnlI MseI MspI MspJI MvnI NdeII RsaI RsaNI
SaqAI Sau3AI SetI SgeI Sse9I SspMI TaiI TaqI
TasI Tru1I Tru9I XspI AclWI AgsI AjnI Alw26I
AlwI AspS9I AsuC2I AsuHPI AvaII BbvI BccI BceAI
BciT130I BcnI BcoDI Bme1390I Bme18I BmgT120I BmrFI BmsI
BpuMI Bse1I BseBI BseGI BseMII BseNI BseXI BslFI
BsmAI BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI
BstF5I BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I
CseI DdeI Eco47I EcoRII FaqI FauI FokI HgaI
HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I LmnI
Lsp1109I LweI MaeIII MboII MlyI MspR9I MvaI NciI
NmuCI PfeI PleI PpsI Psp6I PspGI PspPI Sau96I
SchI ScrFI SfaNI SinI StyD4I TaaI TauI TfiI
TscAI TseFI Tsp45I TspDTI TspGWI TspRI VpaK11BI AanI
AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanI
BanII BauI BbrPI BbsI Bbv12I BciVI BclI BcuI
BfmI BfoI BfrI BfuAI BfuI BglII BlnI BmcAI
BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI BpmI
Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI
Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI
BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI
BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI
Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI
BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I
BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI
BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I
BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I
BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI
BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI
BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I
ClaI CspAI DinI DraI EaeI EagI Eam1104I EarI
EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I
Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI
EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI
Esp3I FauNDI FbaI FblI FriOI FspI GsaI GsuI
HaeII Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III
Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI
KspI MbiI MfeI MflI MhlI MlsI MluI MluNI
Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I
MspCI MunI Mva1269I NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NmeAIII NruI NsbI NsiI NspI NspV
PaeI PaeR7I PagI PauI PceI PciI PctI PdiI
Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I PscI
PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI PspN4I
PspOMI PstI PsuI PteI PvuI PvuII RruI SacI
SacII SalI ScaI SduI SfcI SfoI Sfr274I Sfr303I
SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI SpeI
SphI SseBI SspDI SspI SstI StuI StyI TaqII
TatI TspMI Vha464I VneI VspI XapI XbaI XceI
XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I AarI
AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI
BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I Eco91I
EcoO109I EcoO65I KflI LguI MabI PasI PciSI PfoI
PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI SexAI
AbsI AscI AsiSI CciNI FseI FspAI MauBI MreI
MssI NotI PacI PalAI PmeI PspXI RgaI RigI
SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI SmiI
SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII MteI
PflFI PstNI PsyI Tth111I AleI Asp700I BoxI BsaBI
Bse8I BseJI BstPAI MroXI MslI OliI PdmI PshAI
RseI SmiMI XmnI AccB7I AfiI AhdI BaeI BglI
BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI BstENI
BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI PflMI
Van91I XagI AasI BcgI BstXI CspCI DrdI DseDI
AloI ArsI BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: BisI, AciI, FaeI, SsiI, Hsp92II, NlaIII, Hin1II, SatI, GluI, AbaSI, PkrI, BlsI, TseI, Fsp4HI, CviAII, FaiI, FatI, HpyCH4V, Fnu4HI, ApeKI, MspA1I, BspACI
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 ApeKI TseI
| |
| |7 GluI Fsp4HI Fnu4HI SatI BisI
| ||
| ||8 PkrI BlsI
| |||
ATACGCTGCCACGCA
|||||||||||||||
TATGCGACGGTGCGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviAII CviJI CviKI_1 CviQI DpnI
DpnII FaeI FaiI FatI FspBI GlaI HaeIII HapII
HhaI Hin1II Hin6I HinP1I HpaII HpyCH4IV HpyCH4V HpySE526I
Hsp92II HspAI Kzo9I LpnPI MaeI MaeII MalI MboI
MluCI MnlI MseI MspI MspJI MvnI NdeII NlaIII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI AspS9I AsuC2I AsuHPI AvaII
BbvI BccI BceAI BciT130I BcnI BcoDI Bme1390I Bme18I
BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI BseMII
BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI BsrI
Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI BstV1I
BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII FaqI
FauI FokI HgaI HinfI HphI Hpy188I Hpy99I HpyAV
HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI
MspR9I MvaI NciI NmuCI PfeI PleI PpsI Psp6I
PspGI PspPI Sau96I SchI ScrFI SfaNI SinI StyD4I
TaaI TauI TfiI TscAI TseFI Tsp45I TspDTI TspGWI
TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I
AccBSI AccI AccIII AclI AcoI AcsI AcuI AcvI
AcyI AfeI AflII AflIII AgeI AhlI AjiI Alw21I
Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI
AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI
BalI BamHI BanI BanII BauI BbrPI BbsI Bbv12I
BciVI BclI BcuI BfmI BfoI BfrI BfuAI BfuI
BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI
BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI
BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI
BseMI BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I
BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI
BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I
Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI
BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI
BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I
Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI
BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI
BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI
BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I CciI
Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI
EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I
Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I
Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I
EgeI EheI ErhI Esp3I FauNDI FbaI FblI FriOI
FspI GsaI GsuI HaeII Hin1I HincII HindII HindIII
HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI
KroI Ksp22I KspAI KspI MbiI MfeI MflI MhlI
MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI
MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI
NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI
NsbI NsiI NspI NspV PaeI PaeR7I PagI PauI
PceI PciI PctI PdiI Pfl23II PinAI Ple19I PluTI
PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I
PspCI PspFI PspLI PspN4I PspOMI PstI PsuI PteI
PvuI PvuII RruI SacI SacII SalI ScaI SduI
SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI
SmlI SmoI SnaBI SpeI SphI SseBI SspDI SspI
SstI StuI StyI TaqII TatI TspMI Vha464I VneI
VspI XapI XbaI XceI XhoI XmaI XmaJI XmiI
ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I
Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI
CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI
MabI PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI
Rsr2I RsrII SapI SexAI AbsI AscI AsiSI CciNI
FseI FspAI MauBI MreI MssI NotI PacI PalAI
PmeI PspXI RgaI RigI SbfI SdaI SfaAI SgfI
SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI
AlwNI CaiI DraIII MteI PflFI PstNI PsyI Tth111I
AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI
MslI OliI PdmI PshAI RseI SmiMI XmnI AccB7I
AfiI AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I
BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI
FalI HpyF10VI MwoI PflMI Van91I XagI AasI BcgI
BstXI CspCI DrdI DseDI AloI ArsI BarI PcsI
PsrI SfiI AjuI XcmI
Proposed sequence enzymes: GluI, AbaSI, Fnu4HI, Fsp4HI, SatI, ApeKI, TseI, BisI, BlsI, PkrI
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 MaeII AbaSI HpyCH4IV HpySE526I
|
| 7 TaiI SetI
| |
| |8 TseFI NmuCI Tsp45I MaeIII
| ||
ATACGTTGTCACGCA
|||||||||||||||
TATGCAACAGTGCGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviAII CviJI CviKI_1 CviQI DpnI
DpnII FaeI FaiI FatI FspBI GlaI HaeIII HapII
HhaI Hin1II Hin6I HinP1I HpaII HpyCH4V Hsp92II HspAI
Kzo9I LpnPI MaeI MalI MboI MluCI MnlI MseI
MspI MspJI MvnI NdeII NlaIII RsaI RsaNI SaqAI
Sau3AI SgeI Sse9I SsiI SspMI TaqI TasI Tru1I
Tru9I XspI AclWI AgsI AjnI Alw26I AlwI ApeKI
AspS9I AsuC2I AsuHPI AvaII BbvI BccI BceAI BciT130I
BcnI BcoDI BisI BlsI Bme1390I Bme18I BmgT120I BmrFI
BmsI BpuMI Bse1I BseBI BseGI BseMII BseNI BseXI
BslFI BsmAI BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI
BstDEI BstF5I BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI
Cfr13I CseI DdeI Eco47I EcoRII FaqI FauI Fnu4HI
FokI Fsp4HI GluI HgaI HinfI HphI Hpy188I Hpy99I
HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI MboII MlyI
MspR9I MvaI NciI PfeI PkrI PleI PpsI Psp6I
PspGI PspPI SatI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseI TspDTI TspGWI
TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I
AccBSI AccI AccIII AclI AcoI AcsI AcuI AcvI
AcyI AfeI AflII AflIII AgeI AhlI AjiI Alw21I
Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI
AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI
BalI BamHI BanI BanII BauI BbrPI BbsI Bbv12I
BciVI BclI BcuI BfmI BfoI BfrI BfuAI BfuI
BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI
BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI
BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI
BseMI BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I
BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI
BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I
Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI
BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI
BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I
Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI
BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI
BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI
BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I CciI
Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI
EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I
Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I
Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I
EgeI EheI ErhI Esp3I FauNDI FbaI FblI FriOI
FspI GsaI GsuI HaeII Hin1I HincII HindII HindIII
HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI
KroI Ksp22I KspAI KspI MbiI MfeI MflI MhlI
MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI
MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI
NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI
NsbI NsiI NspI NspV PaeI PaeR7I PagI PauI
PceI PciI PctI PdiI Pfl23II PinAI Ple19I PluTI
PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I
PspCI PspFI PspLI PspN4I PspOMI PstI PsuI PteI
PvuI PvuII RruI SacI SacII SalI ScaI SduI
SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI
SmlI SmoI SnaBI SpeI SphI SseBI SspDI SspI
SstI StuI StyI TaqII TatI TspMI Vha464I VneI
VspI XapI XbaI XceI XhoI XmaI XmaJI XmiI
ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I
Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI
CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI
MabI PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI
Rsr2I RsrII SapI SexAI AbsI AscI AsiSI CciNI
FseI FspAI MauBI MreI MssI NotI PacI PalAI
PmeI PspXI RgaI RigI SbfI SdaI SfaAI SgfI
SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI
AlwNI CaiI DraIII MteI PflFI PstNI PsyI Tth111I
AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI
MslI OliI PdmI PshAI RseI SmiMI XmnI AccB7I
AfiI AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I
BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI
FalI HpyF10VI MwoI PflMI Van91I XagI AasI BcgI
BstXI CspCI DrdI DseDI AloI ArsI BarI PcsI
PsrI SfiI AjuI XcmI
Proposed sequence enzymes: TseFI, MaeII, NmuCI, AbaSI, HpyCH4IV, HpySE526I, Tsp45I, MaeIII, SetI, TaiI
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 ApeKI TseI
| |
| |7 GluI Fsp4HI Fnu4HI SatI BisI
| ||
| ||8 PkrI BlsI
| |||
| ||| 10 FatI
| ||| |
| ||| |11 CviAII
| ||| ||
| ||| ||12 FaiI
| ||| |||
| ||| ||| 14 Hin1II NlaIII FaeI Hsp92II
| ||| ||| |
ATACGCTGCCATGCT
|||||||||||||||
TATGCGACGGTACGA
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI Bme1390I
Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI
BseMII BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI
BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI
BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII
FaqI FauI FokI HgaI HinfI HphI Hpy188I Hpy99I
HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII MboII
MlyI MspR9I MvaI NciI NmuCI PfeI PleI PpsI
Psp6I PspGI PspPI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI Tsp45I TspDTI
TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I
AccB1I AccBSI AccI AccIII AclI AcoI AcsI AcuI
AcvI AcyI AfeI AflII AflIII AgeI AhlI AjiI
Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI
AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII
BaeGI BalI BamHI BanI BanII BauI BbrPI BbsI
Bbv12I BciVI BclI BcuI BfmI BfoI BfrI BfuAI
BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI
BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI
BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI
BseDI BseMI BsePI BseRI BseSI BseX3I BseYI BsgI
Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI
BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I
Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI
BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI
BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI
BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI
BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI
BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI
BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I
CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI
EaeI EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I
Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI
Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I
EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI FblI
FriOI FspI GsaI GsuI HaeII Hin1I HincII HindII
HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I
KpnI KroI Ksp22I KspAI KspI MbiI MfeI MflI
MhlI MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I
MroI MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I
NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII
NruI NsbI NsiI NspI NspV PaeI PaeR7I PagI
PauI PceI PciI PctI PdiI Pfl23II PinAI Ple19I
PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI
Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI PsuI
PteI PvuI PvuII RruI SacI SacII SalI ScaI
SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI
SmaI SmlI SmoI SnaBI SpeI SphI SseBI SspDI
SspI SstI StuI StyI TaqII TatI TspMI Vha464I
VneI VspI XapI XbaI XceI XhoI XmaI XmaJI
XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI
Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I
CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI
LguI MabI PasI PciSI PfoI PpuMI Psp5II PspEI
PspPPI Rsr2I RsrII SapI SexAI AbsI AscI AsiSI
CciNI FseI FspAI MauBI MreI MssI NotI PacI
PalAI PmeI PspXI RgaI RigI SbfI SdaI SfaAI
SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI
AdeI AlwNI CaiI DraIII MteI PflFI PstNI PsyI
Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI
MroXI MslI OliI PdmI PshAI RseI SmiMI XmnI
AccB7I AfiI AhdI BaeI BglI BmeRI BplI BsaXI
Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I
EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI AasI
BcgI BstXI CspCI DrdI DseDI AloI ArsI BarI
PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: TseI, GluI, AbaSI, Fsp4HI, CviAII, FaiI, FaeI, FatI, Fnu4HI, PkrI, SatI, Hsp92II, ApeKI, BisI, BlsI, NlaIII, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 10 FatI
| |
| |11 CviAII
| ||
| ||12 FaiI
| |||
| ||| 14 Hin1II NlaIII FaeI Hsp92II
| ||| |
ATACGATGTCATGCT
|||||||||||||||
TATGCTACAGTACGA
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I
BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI
DdeI Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI
GluI HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III
HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI MspR9I
MvaI NciI NmuCI PfeI PkrI PleI PpsI Psp6I
PspGI PspPI SatI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI TseI Tsp45I
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspA1I MspCI MunI
Mva1269I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV
NmeAIII NruI NsbI NsiI NspI NspV PaeI PaeR7I
PagI PauI PceI PciI PctI PdiI Pfl23II PinAI
Ple19I PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI
Psp124BI Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI
PsuI PteI PvuI PvuII RruI SacI SacII SalI
ScaI SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI
SlaI SmaI SmlI SmoI SnaBI SpeI SphI SseBI
SspDI SspI SstI StuI StyI TaqII TatI TspMI
Vha464I VneI VspI XapI XbaI XceI XhoI XmaI
XmaJI XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI
BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI
Bsu36I CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I
KflI LguI MabI PasI PciSI PfoI PpuMI Psp5II
PspEI PspPPI Rsr2I RsrII SapI SexAI AbsI AscI
AsiSI CciNI FseI FspAI MauBI MreI MssI NotI
PacI PalAI PmeI PspXI RgaI RigI SbfI SdaI
SfaAI SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I
SwaI AdeI AlwNI CaiI DraIII MteI PflFI PstNI
PsyI Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI
BstPAI MroXI MslI OliI PdmI PshAI RseI SmiMI
XmnI AccB7I AfiI AhdI BaeI BglI BmeRI BplI
BsaXI Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI
Eam1105I EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI
AasI BcgI BstXI CspCI DrdI DseDI AloI ArsI
BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 ApeKI TseI
| |
| |7 GluI Fsp4HI Fnu4HI SatI BisI
| ||
| ||8 PkrI BlsI
| |||
| ||| 10 FatI
| ||| |
| ||| |11 CviAII
| ||| ||
| ||| ||12 FaiI
| ||| |||
| ||| ||| 14 Hin1II NlaIII FaeI Hsp92II
| ||| ||| |
ATACGCTGCCATGCC
|||||||||||||||
TATGCGACGGTACGG
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI Bme1390I
Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I BseBI BseGI
BseMII BseNI BseXI BslFI BsmAI BsmFI BspCNI BspPI
BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI
BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII
FaqI FauI FokI HgaI HinfI HphI Hpy188I Hpy99I
HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII MboII
MlyI MspR9I MvaI NciI NmuCI PfeI PleI PpsI
Psp6I PspGI PspPI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI Tsp45I TspDTI
TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I
AccB1I AccBSI AccI AccIII AclI AcoI AcsI AcuI
AcvI AcyI AfeI AflII AflIII AgeI AhlI AjiI
Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI
AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII
BaeGI BalI BamHI BanI BanII BauI BbrPI BbsI
Bbv12I BciVI BclI BcuI BfmI BfoI BfrI BfuAI
BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI
BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI
BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI
BseDI BseMI BsePI BseRI BseSI BseX3I BseYI BsgI
Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI
BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I
Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI
BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI
BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI
BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI
BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI
BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI
BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I
CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI
EaeI EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I
Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI
Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I
EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI FblI
FriOI FspI GsaI GsuI HaeII Hin1I HincII HindII
HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I
KpnI KroI Ksp22I KspAI KspI MbiI MfeI MflI
MhlI MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I
MroI MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I
NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII
NruI NsbI NsiI NspI NspV PaeI PaeR7I PagI
PauI PceI PciI PctI PdiI Pfl23II PinAI Ple19I
PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI
Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI PsuI
PteI PvuI PvuII RruI SacI SacII SalI ScaI
SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI
SmaI SmlI SmoI SnaBI SpeI SphI SseBI SspDI
SspI SstI StuI StyI TaqII TatI TspMI Vha464I
VneI VspI XapI XbaI XceI XhoI XmaI XmaJI
XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI
Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I
CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI
LguI MabI PasI PciSI PfoI PpuMI Psp5II PspEI
PspPPI Rsr2I RsrII SapI SexAI AbsI AscI AsiSI
CciNI FseI FspAI MauBI MreI MssI NotI PacI
PalAI PmeI PspXI RgaI RigI SbfI SdaI SfaAI
SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI
AdeI AlwNI CaiI DraIII MteI PflFI PstNI PsyI
Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI
MroXI MslI OliI PdmI PshAI RseI SmiMI XmnI
AccB7I AfiI AhdI BaeI BglI BmeRI BplI BsaXI
Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I
EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI AasI
BcgI BstXI CspCI DrdI DseDI AloI ArsI BarI
PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: TseI, GluI, AbaSI, Fsp4HI, CviAII, FaiI, FaeI, FatI, Fnu4HI, PkrI, SatI, Hsp92II, ApeKI, BisI, BlsI, NlaIII, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 HpyCH4III Bst4CI TaaI
| |
| | 8 TseFI NmuCI Tsp45I MaeIII
| | |
ATACGGTGTCACGCA
|||||||||||||||
TATGCCACAGTGCGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviAII CviJI CviKI_1 CviQI DpnI
DpnII FaeI FaiI FatI FspBI GlaI HaeIII HapII
HhaI Hin1II Hin6I HinP1I HpaII HpyCH4IV HpyCH4V HpySE526I
Hsp92II HspAI Kzo9I LpnPI MaeI MaeII MalI MboI
MluCI MnlI MseI MspI MspJI MvnI NdeII NlaIII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI BisI
BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I
BseBI BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI
BspCNI BspPI BsrI Bst2UI BstDEI BstF5I BstMAI BstNI
BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI Eco47I
EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI HgaI
HinfI HphI Hpy188I Hpy99I HpyAV HpyF3I LmnI Lsp1109I
LweI MboII MlyI MspR9I MvaI NciI PfeI PkrI
PleI PpsI Psp6I PspGI PspPI SatI Sau96I SchI
ScrFI SfaNI SinI StyD4I TauI TfiI TscAI TseI
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspA1I MspCI MunI
Mva1269I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV
NmeAIII NruI NsbI NsiI NspI NspV PaeI PaeR7I
PagI PauI PceI PciI PctI PdiI Pfl23II PinAI
Ple19I PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI
Psp124BI Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI
PsuI PteI PvuI PvuII RruI SacI SacII SalI
ScaI SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI
SlaI SmaI SmlI SmoI SnaBI SpeI SphI SseBI
SspDI SspI SstI StuI StyI TaqII TatI TspMI
Vha464I VneI VspI XapI XbaI XceI XhoI XmaI
XmaJI XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI
BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI
Bsu36I CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I
KflI LguI MabI PasI PciSI PfoI PpuMI Psp5II
PspEI PspPPI Rsr2I RsrII SapI SexAI AbsI AscI
AsiSI CciNI FseI FspAI MauBI MreI MssI NotI
PacI PalAI PmeI PspXI RgaI RigI SbfI SdaI
SfaAI SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I
SwaI AdeI AlwNI CaiI DraIII MteI PflFI PstNI
PsyI Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI
BstPAI MroXI MslI OliI PdmI PshAI RseI SmiMI
XmnI AccB7I AfiI AhdI BaeI BglI BmeRI BplI
BsaXI Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI
Eam1105I EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI
AasI BcgI BstXI CspCI DrdI DseDI AloI ArsI
BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: TseFI, AbaSI, Tsp45I, NmuCI, Bst4CI, TaaI, MaeIII, HpyCH4III
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 10 FatI
| |
| |11 CviAII
| ||
| ||12 FaiI
| |||
| ||| 14 Hin1II NlaIII FaeI Hsp92II
| ||| |
ATACGATGTCATGCC
|||||||||||||||
TATGCTACAGTACGG
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I
BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI
DdeI Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI
GluI HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III
HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI MspR9I
MvaI NciI NmuCI PfeI PkrI PleI PpsI Psp6I
PspGI PspPI SatI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI TseI Tsp45I
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspA1I MspCI MunI
Mva1269I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV
NmeAIII NruI NsbI NsiI NspI NspV PaeI PaeR7I
PagI PauI PceI PciI PctI PdiI Pfl23II PinAI
Ple19I PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI
Psp124BI Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI
PsuI PteI PvuI PvuII RruI SacI SacII SalI
ScaI SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI
SlaI SmaI SmlI SmoI SnaBI SpeI SphI SseBI
SspDI SspI SstI StuI StyI TaqII TatI TspMI
Vha464I VneI VspI XapI XbaI XceI XhoI XmaI
XmaJI XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI
BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI
Bsu36I CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I
KflI LguI MabI PasI PciSI PfoI PpuMI Psp5II
PspEI PspPPI Rsr2I RsrII SapI SexAI AbsI AscI
AsiSI CciNI FseI FspAI MauBI MreI MssI NotI
PacI PalAI PmeI PspXI RgaI RigI SbfI SdaI
SfaAI SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I
SwaI AdeI AlwNI CaiI DraIII MteI PflFI PstNI
PsyI Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI
BstPAI MroXI MslI OliI PdmI PshAI RseI SmiMI
XmnI AccB7I AfiI AhdI BaeI BglI BmeRI BplI
BsaXI Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI
Eam1105I EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI
AasI BcgI BstXI CspCI DrdI DseDI AloI ArsI
BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 AbaSI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
ATACGCTGTCATGCG
|||||||||||||||
TATGCGACAGTACGC
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I
BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI
DdeI Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI
GluI HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III
HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI MspR9I
MvaI NciI NmuCI PfeI PkrI PleI PpsI Psp6I
PspGI PspPI SatI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI TseI Tsp45I
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspA1I MspCI MunI
Mva1269I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV
NmeAIII NruI NsbI NsiI NspI NspV PaeI PaeR7I
PagI PauI PceI PciI PctI PdiI Pfl23II PinAI
Ple19I PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI
Psp124BI Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI
PsuI PteI PvuI PvuII RruI SacI SacII SalI
ScaI SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI
SlaI SmaI SmlI SmoI SnaBI SpeI SphI SseBI
SspDI SspI SstI StuI StyI TaqII TatI TspMI
Vha464I VneI VspI XapI XbaI XceI XhoI XmaI
XmaJI XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI
BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI
Bsu36I CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I
KflI LguI MabI PasI PciSI PfoI PpuMI Psp5II
PspEI PspPPI Rsr2I RsrII SapI SexAI AbsI AscI
AsiSI CciNI FseI FspAI MauBI MreI MssI NotI
PacI PalAI PmeI PspXI RgaI RigI SbfI SdaI
SfaAI SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I
SwaI AdeI AlwNI CaiI DraIII MteI PflFI PstNI
PsyI Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI
BstPAI MroXI MslI OliI PdmI PshAI RseI SmiMI
XmnI AccB7I AfiI AhdI BaeI BglI BmeRI BplI
BsaXI Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI
Eam1105I EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI
AasI BcgI BstXI CspCI DrdI DseDI AloI ArsI
BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 10 FatI
| |
| |11 CviAII
| ||
| ||12 FaiI
| |||
| ||| 14 Hin1II NlaIII FaeI Hsp92II
| ||| |
ATACGCTGTCATGCC
|||||||||||||||
TATGCGACAGTACGG
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I
BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI
DdeI Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI
GluI HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III
HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI MspR9I
MvaI NciI NmuCI PfeI PkrI PleI PpsI Psp6I
PspGI PspPI SatI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI TseI Tsp45I
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspA1I MspCI MunI
Mva1269I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV
NmeAIII NruI NsbI NsiI NspI NspV PaeI PaeR7I
PagI PauI PceI PciI PctI PdiI Pfl23II PinAI
Ple19I PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI
Psp124BI Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI
PsuI PteI PvuI PvuII RruI SacI SacII SalI
ScaI SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI
SlaI SmaI SmlI SmoI SnaBI SpeI SphI SseBI
SspDI SspI SstI StuI StyI TaqII TatI TspMI
Vha464I VneI VspI XapI XbaI XceI XhoI XmaI
XmaJI XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI
BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI
Bsu36I CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I
KflI LguI MabI PasI PciSI PfoI PpuMI Psp5II
PspEI PspPPI Rsr2I RsrII SapI SexAI AbsI AscI
AsiSI CciNI FseI FspAI MauBI MreI MssI NotI
PacI PalAI PmeI PspXI RgaI RigI SbfI SdaI
SfaAI SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I
SwaI AdeI AlwNI CaiI DraIII MteI PflFI PstNI
PsyI Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI
BstPAI MroXI MslI OliI PdmI PshAI RseI SmiMI
XmnI AccB7I AfiI AhdI BaeI BglI BmeRI BplI
BsaXI Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI
Eam1105I EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI
AasI BcgI BstXI CspCI DrdI DseDI AloI ArsI
BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
|5 Hpy188I
||
|| 10 FatI
|| |
|| |11 CviAII
|| ||
|| ||12 FaiI
|| |||
|| ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
|| ||| |
|| ||| |15 AbaSI
|| ||| ||
ATCCGATGTCATGCA
|||||||||||||||
TAGGCTACAGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpySE526I HspAI Kzo9I LpnPI MaeI MaeII MalI
MboI MluCI MnlI MseI MspI MspJI MvnI NdeII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI BisI
BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I
BseBI BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI
BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI
BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI
Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI
HgaI HinfI HphI Hpy99I HpyAV HpyCH4III HpyF3I LmnI
Lsp1109I LweI MaeIII MboII MlyI MspR9I MvaI NciI
NmuCI PfeI PkrI PleI PpsI Psp6I PspGI PspPI
SatI Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI
TauI TfiI TscAI TseFI TseI Tsp45I TspDTI TspGWI
TspRI VpaK11BI AanI AatII Acc16I Acc36I Acc65I AccB1I
AccBSI AccI AccIII AclI AcoI AcsI AcuI AcvI
AcyI AfeI AflII AflIII AgeI AhlI AjiI Alw21I
Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI ApoI AseI
AsiGI Asp718I AspA2I AsuII AsuNHI AvaI AvrII BaeGI
BalI BamHI BanI BanII BauI BbrPI BbsI Bbv12I
BciVI BclI BcuI BfmI BfoI BfrI BfuAI BfuI
BglII BlnI BmcAI BmeT110I BmgBI BmiI BmrI BmtI
BmuI BpiI BpmI Bpu14I BpuEI Bsa29I BsaAI BsaHI
BsaI BsaJI BsaWI Bse118I Bse3DI BseAI BseCI BseDI
BseMI BsePI BseRI BseSI BseX3I BseYI BsgI Bsh1285I
BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI BsiWI BsmBI
BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I
Bsp19I Bsp68I BspDI BspEI BspHI BspLI BspMAI BspMI
BspOI BspT104I BspT107I BspTI BspTNI BsrBI BsrDI BsrFI
BsrGI BssAI BssECI BssHII BssNAI BssNI BssSI BssT1I
Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI BstBAI BstBI
BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI BstSLI BstSNI
BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I BsuI BsuTUI
BtgI BtgZI BtrI BtsI BtuMI BveI Cac8I CciI
Cfr10I Cfr42I Cfr9I ClaI CspAI DinI DraI EaeI
EagI Eam1104I EarI EciI Ecl136II EclXI Eco105I Eco130I
Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I Eco53kI Eco57I
Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I EcoT22I EcoT38I
EgeI EheI ErhI Esp3I FauNDI FbaI FblI FriOI
FspI GsaI GsuI HaeII Hin1I HincII HindII HindIII
HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI Kpn2I KpnI
KroI Ksp22I KspAI KspI MbiI MfeI MflI MhlI
MlsI MluI MluNI Mly113I MmeI Mox20I Mph1103I MroI
MroNI MscI Msp20I MspA1I MspCI MunI Mva1269I NaeI
NarI NcoI NdeI NgoMIV NheI NlaIV NmeAIII NruI
NsbI NsiI NspI NspV PaeI PaeR7I PagI PauI
PceI PciI PctI PdiI Pfl23II PinAI Ple19I PluTI
PmaCI PmlI Ppu21I PscI PshBI PsiI Psp124BI Psp1406I
PspCI PspFI PspLI PspN4I PspOMI PstI PsuI PteI
PvuI PvuII RruI SacI SacII SalI ScaI SduI
SfcI SfoI Sfr274I Sfr303I SfuI SgrBI SlaI SmaI
SmlI SmoI SnaBI SpeI SphI SseBI SspDI SspI
SstI StuI StyI TaqII TatI TspMI Vha464I VneI
VspI XapI XbaI XceI XhoI XmaI XmaJI XmiI
ZraI ZrmI Zsp2I AarI AxyI BbvCI BlpI Bpu10I
Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI Bsu36I CpoI
CsiI CspI Eco81I Eco91I EcoO109I EcoO65I KflI LguI
MabI PasI PciSI PfoI PpuMI Psp5II PspEI PspPPI
Rsr2I RsrII SapI SexAI AbsI AscI AsiSI CciNI
FseI FspAI MauBI MreI MssI NotI PacI PalAI
PmeI PspXI RgaI RigI SbfI SdaI SfaAI SgfI
SgrAI SgrDI SgsI SmiI SrfI Sse8387I SwaI AdeI
AlwNI CaiI DraIII MteI PflFI PstNI PsyI Tth111I
AleI Asp700I BoxI BsaBI Bse8I BseJI BstPAI MroXI
MslI OliI PdmI PshAI RseI SmiMI XmnI AccB7I
AfiI AhdI BaeI BglI BmeRI BplI BsaXI Bsc4I
BseLI BslI BstAPI BstENI BstMWI DriI Eam1105I EcoNI
FalI HpyF10VI MwoI PflMI Van91I XagI AasI BcgI
BstXI CspCI DrdI DseDI AloI ArsI BarI PcsI
PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, HpyCH4V, Hsp92II, Hpy188I, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 MaeII AbaSI HpyCH4IV HpySE526I
|
| 7 TaiI SetI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI HpyCH4V Hsp92II
| | ||| |
ATACGTTGTCATGCA
|||||||||||||||
TATGCAACAGTACGT
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HspAI Kzo9I LpnPI MaeI MalI MboI MluCI MnlI
MseI MspI MspJI MvnI NdeII RsaI RsaNI SaqAI
Sau3AI SgeI Sse9I SsiI SspMI TaqI TasI Tru1I
Tru9I XspI AclWI AgsI AjnI Alw26I AlwI ApeKI
AspS9I AsuC2I AsuHPI AvaII BbvI BccI BceAI BciT130I
BcnI BcoDI BisI BlsI Bme1390I Bme18I BmgT120I BmrFI
BmsI BpuMI Bse1I BseBI BseGI BseMII BseNI BseXI
BslFI BsmAI BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI
BstDEI BstF5I BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI
Cfr13I CseI DdeI Eco47I EcoRII FaqI FauI Fnu4HI
FokI Fsp4HI GluI HgaI HinfI HphI Hpy188I Hpy99I
HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII MboII
MlyI MspR9I MvaI NciI NmuCI PfeI PkrI PleI
PpsI Psp6I PspGI PspPI SatI Sau96I SchI ScrFI
SfaNI SinI StyD4I TaaI TauI TfiI TscAI TseFI
TseI Tsp45I TspDTI TspGWI TspRI VpaK11BI AanI AatII
Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII AclI
AcoI AcsI AcuI AcvI AcyI AfeI AflII AflIII
AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI
ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I AsuII
AsuNHI AvaI AvrII BaeGI BalI BamHI BanI BanII
BauI BbrPI BbsI Bbv12I BciVI BclI BcuI BfmI
BfoI BfrI BfuAI BfuI BglII BlnI BmcAI BmeT110I
BmgBI BmiI BmrI BmtI BmuI BpiI BpmI Bpu14I
BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI Bse118I
Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI BseSI
BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI BsiEI
BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I
Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI
BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I BspTI
BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI BssHII
BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI
BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I BstMCI
BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I
BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI BtsI
BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI
CspAI DinI DraI EaeI EagI Eam1104I EarI EciI
Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I
Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI
EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI Esp3I
FauNDI FbaI FblI FriOI FspI GsaI GsuI HaeII
Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III Hpy8I
Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI KspI
MbiI MfeI MflI MhlI MlsI MluI MluNI Mly113I
MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I MspA1I
MspCI MunI Mva1269I NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NmeAIII NruI NsbI NsiI NspI NspV
PaeI PaeR7I PagI PauI PceI PciI PctI PdiI
Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I PscI
PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI PspN4I
PspOMI PstI PsuI PteI PvuI PvuII RruI SacI
SacII SalI ScaI SduI SfcI SfoI Sfr274I Sfr303I
SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI SpeI
SphI SseBI SspDI SspI SstI StuI StyI TaqII
TatI TspMI Vha464I VneI VspI XapI XbaI XceI
XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I AarI
AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI
BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I Eco91I
EcoO109I EcoO65I KflI LguI MabI PasI PciSI PfoI
PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI SexAI
AbsI AscI AsiSI CciNI FseI FspAI MauBI MreI
MssI NotI PacI PalAI PmeI PspXI RgaI RigI
SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI SmiI
SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII MteI
PflFI PstNI PsyI Tth111I AleI Asp700I BoxI BsaBI
Bse8I BseJI BstPAI MroXI MslI OliI PdmI PshAI
RseI SmiMI XmnI AccB7I AfiI AhdI BaeI BglI
BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI BstENI
BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI PflMI
Van91I XagI AasI BcgI BstXI CspCI DrdI DseDI
AloI ArsI BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: MaeII, NlaIII, AbaSI, CviAII, HpyCH4IV, TaiI, FaeI, HpySE526I, FaiI, FatI, Hsp92II, HpyCH4V, SetI, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 6 AbaSI
| |
| | 10 FatI
| | |
| | |11 CviAII
| | ||
| | ||12 FaiI
| | |||
| | ||| 14 Hin1II NlaIII FaeI Hsp92II
| | ||| |
ATTCGCTGTCATGCG
|||||||||||||||
TAAGCGACAGTACGC
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4IV HpyCH4V HpySE526I HspAI Kzo9I LpnPI MaeI MaeII
MalI MboI MluCI MnlI MseI MspI MspJI MvnI
NdeII RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I
SsiI SspMI TaiI TaqI TasI Tru1I Tru9I XspI
AclWI AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I
AsuHPI AvaII BbvI BccI BceAI BciT130I BcnI BcoDI
BisI BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI
Bse1I BseBI BseGI BseMII BseNI BseXI BslFI BsmAI
BsmFI BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I
BstMAI BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI
DdeI Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI
GluI HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III
HpyF3I LmnI Lsp1109I LweI MaeIII MboII MlyI MspR9I
MvaI NciI NmuCI PfeI PkrI PleI PpsI Psp6I
PspGI PspPI SatI Sau96I SchI ScrFI SfaNI SinI
StyD4I TaaI TauI TfiI TscAI TseFI TseI Tsp45I
TspDTI TspGWI TspRI VpaK11BI AanI AatII Acc16I Acc36I
Acc65I AccB1I AccBSI AccI AccIII AclI AcoI AcsI
AcuI AcvI AcyI AfeI AflII AflIII AgeI AhlI
AjiI Alw21I Alw44I Ama87I Aor13HI Aor51HI ApaI ApaLI
ApoI AseI AsiGI Asp718I AspA2I AsuII AsuNHI AvaI
AvrII BaeGI BalI BamHI BanI BanII BauI BbrPI
BbsI Bbv12I BciVI BclI BcuI BfmI BfoI BfrI
BfuAI BfuI BglII BlnI BmcAI BmeT110I BmgBI BmiI
BmrI BmtI BmuI BpiI BpmI Bpu14I BpuEI Bsa29I
BsaAI BsaHI BsaI BsaJI BsaWI Bse118I Bse3DI BseAI
BseCI BseDI BseMI BsePI BseRI BseSI BseX3I BseYI
BsgI Bsh1285I BshNI BshTI BshVI BsiEI BsiHKAI BsiHKCI
BsiWI BsmBI BsmI Bso31I BsoBI Bsp119I Bsp120I Bsp1286I
Bsp13I Bsp1407I Bsp19I Bsp68I BspDI BspEI BspHI BspLI
BspMAI BspMI BspOI BspT104I BspT107I BspTI BspTNI BsrBI
BsrDI BsrFI BsrGI BssAI BssECI BssHII BssNAI BssNI
BssSI BssT1I Bst1107I Bst2BI Bst6I BstACI BstAFI BstAUI
BstBAI BstBI BstC8I BstDSI BstH2I BstMCI BstNSI BstSFI
BstSLI BstSNI BstV2I BstX2I BstYI BstZ17I BstZI Bsu15I
BsuI BsuTUI BtgI BtgZI BtrI BtsI BtuMI BveI
Cac8I CciI Cfr10I Cfr42I Cfr9I ClaI CspAI DinI
DraI EaeI EagI Eam1104I EarI EciI Ecl136II EclXI
Eco105I Eco130I Eco147I Eco24I Eco31I Eco32I Eco47III Eco52I
Eco53kI Eco57I Eco72I Eco88I EcoICRI EcoRI EcoRV EcoT14I
EcoT22I EcoT38I EgeI EheI ErhI Esp3I FauNDI FbaI
FblI FriOI FspI GsaI GsuI HaeII Hin1I HincII
HindII HindIII HpaI Hpy166II Hpy188III Hpy8I Hsp92I KasI
Kpn2I KpnI KroI Ksp22I KspAI KspI MbiI MfeI
MflI MhlI MlsI MluI MluNI Mly113I MmeI Mox20I
Mph1103I MroI MroNI MscI Msp20I MspA1I MspCI MunI
Mva1269I NaeI NarI NcoI NdeI NgoMIV NheI NlaIV
NmeAIII NruI NsbI NsiI NspI NspV PaeI PaeR7I
PagI PauI PceI PciI PctI PdiI Pfl23II PinAI
Ple19I PluTI PmaCI PmlI Ppu21I PscI PshBI PsiI
Psp124BI Psp1406I PspCI PspFI PspLI PspN4I PspOMI PstI
PsuI PteI PvuI PvuII RruI SacI SacII SalI
ScaI SduI SfcI SfoI Sfr274I Sfr303I SfuI SgrBI
SlaI SmaI SmlI SmoI SnaBI SpeI SphI SseBI
SspDI SspI SstI StuI StyI TaqII TatI TspMI
Vha464I VneI VspI XapI XbaI XceI XhoI XmaI
XmaJI XmiI ZraI ZrmI Zsp2I AarI AxyI BbvCI
BlpI Bpu10I Bpu1102I Bse21I Bsp1720I BspQI BstEII BstPI
Bsu36I CpoI CsiI CspI Eco81I Eco91I EcoO109I EcoO65I
KflI LguI MabI PasI PciSI PfoI PpuMI Psp5II
PspEI PspPPI Rsr2I RsrII SapI SexAI AbsI AscI
AsiSI CciNI FseI FspAI MauBI MreI MssI NotI
PacI PalAI PmeI PspXI RgaI RigI SbfI SdaI
SfaAI SgfI SgrAI SgrDI SgsI SmiI SrfI Sse8387I
SwaI AdeI AlwNI CaiI DraIII MteI PflFI PstNI
PsyI Tth111I AleI Asp700I BoxI BsaBI Bse8I BseJI
BstPAI MroXI MslI OliI PdmI PshAI RseI SmiMI
XmnI AccB7I AfiI AhdI BaeI BglI BmeRI BplI
BsaXI Bsc4I BseLI BslI BstAPI BstENI BstMWI DriI
Eam1105I EcoNI FalI HpyF10VI MwoI PflMI Van91I XagI
AasI BcgI BstXI CspCI DrdI DseDI AloI ArsI
BarI PcsI PsrI SfiI AjuI XcmI
Proposed sequence enzymes: AbaSI, NlaIII, CviAII, FaiI, FatI, FaeI, Hsp92II, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 MaeII AbaSI HpyCH4IV HpySE526I
|
| 6 AbaSI
| |
| |7 TaiI SetI
| ||
| || 10 FatI
| || |
| || |11 CviAII
| || ||
| || ||12 FaiI
| || |||
| || ||| 14 Hin1II NlaIII FaeI Hsp92II
| || ||| |
ATACGTTGTCATGCG
|||||||||||||||
TATGCAACAGTACGC
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviJI CviKI_1 CviQI DpnI DpnII
FspBI GlaI HaeIII HapII HhaI Hin6I HinP1I HpaII
HpyCH4V HspAI Kzo9I LpnPI MaeI MalI MboI MluCI
MnlI MseI MspI MspJI MvnI NdeII RsaI RsaNI
SaqAI Sau3AI SgeI Sse9I SsiI SspMI TaqI TasI
Tru1I Tru9I XspI AclWI AgsI AjnI Alw26I AlwI
ApeKI AspS9I AsuC2I AsuHPI AvaII BbvI BccI BceAI
BciT130I BcnI BcoDI BisI BlsI Bme1390I Bme18I BmgT120I
BmrFI BmsI BpuMI Bse1I BseBI BseGI BseMII BseNI
BseXI BslFI BsmAI BsmFI BspCNI BspPI BsrI Bst2UI
Bst4CI BstDEI BstF5I BstMAI BstNI BstSCI BstV1I BtsCI
BtsIMutI Cfr13I CseI DdeI Eco47I EcoRII FaqI FauI
Fnu4HI FokI Fsp4HI GluI HgaI HinfI HphI Hpy188I
Hpy99I HpyAV HpyCH4III HpyF3I LmnI Lsp1109I LweI MaeIII
MboII MlyI MspR9I MvaI NciI NmuCI PfeI PkrI
PleI PpsI Psp6I PspGI PspPI SatI Sau96I SchI
ScrFI SfaNI SinI StyD4I TaaI TauI TfiI TscAI
TseFI TseI Tsp45I TspDTI TspGWI TspRI VpaK11BI AanI
AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanI
BanII BauI BbrPI BbsI Bbv12I BciVI BclI BcuI
BfmI BfoI BfrI BfuAI BfuI BglII BlnI BmcAI
BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI BpmI
Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI
Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI
BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI
BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI
Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI
BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I
BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI
BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I
BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I
BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI
BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI
BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I
ClaI CspAI DinI DraI EaeI EagI Eam1104I EarI
EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I
Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI
EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI
Esp3I FauNDI FbaI FblI FriOI FspI GsaI GsuI
HaeII Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III
Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI
KspI MbiI MfeI MflI MhlI MlsI MluI MluNI
Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I
MspA1I MspCI MunI Mva1269I NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI NspI
NspV PaeI PaeR7I PagI PauI PceI PciI PctI
PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I
PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI
PspN4I PspOMI PstI PsuI PteI PvuI PvuII RruI
SacI SacII SalI ScaI SduI SfcI SfoI Sfr274I
Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI
SpeI SphI SseBI SspDI SspI SstI StuI StyI
TaqII TatI TspMI Vha464I VneI VspI XapI XbaI
XceI XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I
AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I
BspQI BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I
Eco91I EcoO109I EcoO65I KflI LguI MabI PasI PciSI
PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI
SexAI AbsI AscI AsiSI CciNI FseI FspAI MauBI
MreI MssI NotI PacI PalAI PmeI PspXI RgaI
RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI
SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII
MteI PflFI PstNI PsyI Tth111I AleI Asp700I BoxI
BsaBI Bse8I BseJI BstPAI MroXI MslI OliI PdmI
PshAI RseI SmiMI XmnI AccB7I AfiI AhdI BaeI
BglI BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI
BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI
PflMI Van91I XagI AasI BcgI BstXI CspCI DrdI
DseDI AloI ArsI BarI PcsI PsrI SfiI AjuI
XcmI
Proposed sequence enzymes: MaeII, NlaIII, AbaSI, CviAII, HpyCH4IV, TaiI, FaeI, HpySE526I, FaiI, FatI, Hsp92II, SetI, Hin1II
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Original sequence enzymes: Hin1II, NlaIII, FaeI, AbaSI, CviAII, FaiI, FatI, HpyCH4V, Hsp92II
4 AbaSI
|
| 8 TseFI NmuCI Tsp45I MaeIII
| |
ATACGCTGTCACGCC
|||||||||||||||
TATGCGACAGTGCGG
1 15
Enzymes which do not cut the sequence.
FspEI AccII AciI AfaI AluBI AluI AoxI AspLEI
BfaI BfuCI Bsh1236I BshFI BsiSI BsnI Bsp143I BspACI
BspANI BspFNI BssMI BstFNI BstHHI BstKTI BstMBI BstUI
BsuRI CfoI Csp6I CviAII CviJI CviKI_1 CviQI DpnI
DpnII FaeI FaiI FatI FspBI GlaI HaeIII HapII
HhaI Hin1II Hin6I HinP1I HpaII HpyCH4IV HpyCH4V HpySE526I
Hsp92II HspAI Kzo9I LpnPI MaeI MaeII MalI MboI
MluCI MnlI MseI MspI MspJI MvnI NdeII NlaIII
RsaI RsaNI SaqAI Sau3AI SetI SgeI Sse9I SsiI
SspMI TaiI TaqI TasI Tru1I Tru9I XspI AclWI
AgsI AjnI Alw26I AlwI ApeKI AspS9I AsuC2I AsuHPI
AvaII BbvI BccI BceAI BciT130I BcnI BcoDI BisI
BlsI Bme1390I Bme18I BmgT120I BmrFI BmsI BpuMI Bse1I
BseBI BseGI BseMII BseNI BseXI BslFI BsmAI BsmFI
BspCNI BspPI BsrI Bst2UI Bst4CI BstDEI BstF5I BstMAI
BstNI BstSCI BstV1I BtsCI BtsIMutI Cfr13I CseI DdeI
Eco47I EcoRII FaqI FauI Fnu4HI FokI Fsp4HI GluI
HgaI HinfI HphI Hpy188I Hpy99I HpyAV HpyCH4III HpyF3I
LmnI Lsp1109I LweI MboII MlyI MspR9I MvaI NciI
PfeI PkrI PleI PpsI Psp6I PspGI PspPI SatI
Sau96I SchI ScrFI SfaNI SinI StyD4I TaaI TauI
TfiI TscAI TseI TspDTI TspGWI TspRI VpaK11BI AanI
AatII Acc16I Acc36I Acc65I AccB1I AccBSI AccI AccIII
AclI AcoI AcsI AcuI AcvI AcyI AfeI AflII
AflIII AgeI AhlI AjiI Alw21I Alw44I Ama87I Aor13HI
Aor51HI ApaI ApaLI ApoI AseI AsiGI Asp718I AspA2I
AsuII AsuNHI AvaI AvrII BaeGI BalI BamHI BanI
BanII BauI BbrPI BbsI Bbv12I BciVI BclI BcuI
BfmI BfoI BfrI BfuAI BfuI BglII BlnI BmcAI
BmeT110I BmgBI BmiI BmrI BmtI BmuI BpiI BpmI
Bpu14I BpuEI Bsa29I BsaAI BsaHI BsaI BsaJI BsaWI
Bse118I Bse3DI BseAI BseCI BseDI BseMI BsePI BseRI
BseSI BseX3I BseYI BsgI Bsh1285I BshNI BshTI BshVI
BsiEI BsiHKAI BsiHKCI BsiWI BsmBI BsmI Bso31I BsoBI
Bsp119I Bsp120I Bsp1286I Bsp13I Bsp1407I Bsp19I Bsp68I BspDI
BspEI BspHI BspLI BspMAI BspMI BspOI BspT104I BspT107I
BspTI BspTNI BsrBI BsrDI BsrFI BsrGI BssAI BssECI
BssHII BssNAI BssNI BssSI BssT1I Bst1107I Bst2BI Bst6I
BstACI BstAFI BstAUI BstBAI BstBI BstC8I BstDSI BstH2I
BstMCI BstNSI BstSFI BstSLI BstSNI BstV2I BstX2I BstYI
BstZ17I BstZI Bsu15I BsuI BsuTUI BtgI BtgZI BtrI
BtsI BtuMI BveI Cac8I CciI Cfr10I Cfr42I Cfr9I
ClaI CspAI DinI DraI EaeI EagI Eam1104I EarI
EciI Ecl136II EclXI Eco105I Eco130I Eco147I Eco24I Eco31I
Eco32I Eco47III Eco52I Eco53kI Eco57I Eco72I Eco88I EcoICRI
EcoRI EcoRV EcoT14I EcoT22I EcoT38I EgeI EheI ErhI
Esp3I FauNDI FbaI FblI FriOI FspI GsaI GsuI
HaeII Hin1I HincII HindII HindIII HpaI Hpy166II Hpy188III
Hpy8I Hsp92I KasI Kpn2I KpnI KroI Ksp22I KspAI
KspI MbiI MfeI MflI MhlI MlsI MluI MluNI
Mly113I MmeI Mox20I Mph1103I MroI MroNI MscI Msp20I
MspA1I MspCI MunI Mva1269I NaeI NarI NcoI NdeI
NgoMIV NheI NlaIV NmeAIII NruI NsbI NsiI NspI
NspV PaeI PaeR7I PagI PauI PceI PciI PctI
PdiI Pfl23II PinAI Ple19I PluTI PmaCI PmlI Ppu21I
PscI PshBI PsiI Psp124BI Psp1406I PspCI PspFI PspLI
PspN4I PspOMI PstI PsuI PteI PvuI PvuII RruI
SacI SacII SalI ScaI SduI SfcI SfoI Sfr274I
Sfr303I SfuI SgrBI SlaI SmaI SmlI SmoI SnaBI
SpeI SphI SseBI SspDI SspI SstI StuI StyI
TaqII TatI TspMI Vha464I VneI VspI XapI XbaI
XceI XhoI XmaI XmaJI XmiI ZraI ZrmI Zsp2I
AarI AxyI BbvCI BlpI Bpu10I Bpu1102I Bse21I Bsp1720I
BspQI BstEII BstPI Bsu36I CpoI CsiI CspI Eco81I
Eco91I EcoO109I EcoO65I KflI LguI MabI PasI PciSI
PfoI PpuMI Psp5II PspEI PspPPI Rsr2I RsrII SapI
SexAI AbsI AscI AsiSI CciNI FseI FspAI MauBI
MreI MssI NotI PacI PalAI PmeI PspXI RgaI
RigI SbfI SdaI SfaAI SgfI SgrAI SgrDI SgsI
SmiI SrfI Sse8387I SwaI AdeI AlwNI CaiI DraIII
MteI PflFI PstNI PsyI Tth111I AleI Asp700I BoxI
BsaBI Bse8I BseJI BstPAI MroXI MslI OliI PdmI
PshAI RseI SmiMI XmnI AccB7I AfiI AhdI BaeI
BglI BmeRI BplI BsaXI Bsc4I BseLI BslI BstAPI
BstENI BstMWI DriI Eam1105I EcoNI FalI HpyF10VI MwoI
PflMI Van91I XagI AasI BcgI BstXI CspCI DrdI
DseDI AloI ArsI BarI PcsI PsrI SfiI AjuI
XcmI
Proposed sequence enzymes: TseFI, AbaSI, MaeIII, Tsp45I, NmuCI
Enzimes only in original sequence:
Enzimes only in proposed sequence:
=========================
Content source: Serulab/Py4Bio
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